Departments of Oncology, Ruijin Hospital, Medical School of Shanghai, Jiaotong University, Shanghai, People's Republic of China.
J Surg Oncol. 2011 Feb;103(2):110-5. doi: 10.1002/jso.21777. Epub 2010 Dec 22.
The present study was designed to investigate the clinicopathological role of survivin-expressing circulating tumor cells (CTCs) and to determine whether the presence of survivin-expressing CTCs is an independent predictor of tumor recurrence following curative resection of gastric cancer.
This study included 98 patients who underwent potentially curative resection. Reverse transcription polymerase chain reaction enzyme linked immunosorbent assay (RT-PCR ELISA) was used to measure survivin mRNA in peripheral blood.
Of the 98 patients studied, 45 (45.9%) were positive for survivin mRNA. Survivin mRNA expression correlated with Lauren classification (P < 0.001), pathological tumor (pT) stage (P < 0.001), pathological tumor node metastasis (pTNM) stage (P = 0.009), and degree of differentiation (P = 0.001). The pTNM stage and the status of survivin mRNA were independent prognostic factors of disease-free survival (P = 0.007 and <0.001, respectively).
The detection of CTCs expressing survivin mRNA could be a good clinical biomarker used to predict the prognosis of patients with curatively resected gastric cancer.
本研究旨在探讨存活素表达循环肿瘤细胞（CTCs）的临床病理作用，并确定存活素表达 CTCs 是否是胃癌根治性切除术后肿瘤复发的独立预测因子。
本研究纳入了 98 例接受潜在根治性切除术的患者。采用逆转录聚合酶链反应酶联免疫吸附试验（RT-PCR ELISA）检测外周血中存活素 mRNA。
在研究的 98 例患者中，有 45 例（45.9%）存活素 mRNA 阳性。存活素 mRNA 表达与 Lauren 分类（P < 0.001）、病理肿瘤（pT）分期（P < 0.001）、病理肿瘤淋巴结转移（pTNM）分期（P = 0.009）和分化程度（P = 0.001）相关。pTNM 分期和存活素 mRNA 状态是无病生存的独立预后因素（P = 0.007 和 <0.001）。
检测表达存活素 mRNA 的 CTCs 可能是预测可根治性切除的胃癌患者预后的良好临床生物标志物。
