Sekiguchi I, Suzuki M, Izumi A, Aida I, Tamada T
Department of Obstetrics and Gynecology, Jichi Medical School.
Nihon Gan Chiryo Gakkai Shi. 1990 Nov 20;25(11):2659-64.
To investigate the immunological effect of Sizofilan (SPG) combined with radiotherapy, we evaluated the immunological parameters in 22 patients with uterine cervical cancers. Twelve cases were treated with SPG combined with radiotherapy (SPG group), and the other ten cases, with radiotherapy only (control group). As a result, 1) During radiotherapy, the numbers of lymphocyte and CD2 positive cell decreased in SPG and control groups. After radiotherapy, however, its numbers in SPG group became significantly higher than in control group (p less than 0.05). The number of CD3 positive cell also presented a tendency to increase after radiotherapy in SPG group. As for CD20 positive cell, its numbers were kept unchanged after radiotherapy in both two groups, and no significant difference was observed between them. 2) NK cell activity decreased during radiotherapy in both two groups. After radiotherapy, its activity in SPG group recovered to its pre-value and became significantly higher than that in control group (p less than 0.05). 3) SPG did not any prominent effect on CD4/CD8 ratio. 4) The adverse effect of SPG to liver or kidney function were not observed in our patients. The SCC level in SPG group decreased rapidly by radiotherapy as well as that in control group, and no significant difference was observed in SCC levels between them. So it was suggested that SPG did not suppress the cytocidal effect of radiation to cancer cells. Based on these findings, it was concluded that SPG prompted the recovery of not only lymphocyte, especially T cell, but also NK cell activity. These immunological findings presented a usefulness of clinical application of SPG to radiotherapy in patients with uterine cervical cancers.
为研究裂褶菌多糖（SPG）联合放疗的免疫效应，我们评估了22例子宫颈癌患者的免疫参数。12例患者接受SPG联合放疗（SPG组），另外10例仅接受放疗（对照组）。结果如下：1）放疗期间，SPG组和对照组的淋巴细胞及CD2阳性细胞数量均减少。然而，放疗后，SPG组的这些细胞数量显著高于对照组（p<0.05）。SPG组放疗后CD3阳性细胞数量也有增加趋势。至于CD20阳性细胞，两组放疗后其数量均保持不变，且两组间无显著差异。2）两组放疗期间NK细胞活性均降低。放疗后，SPG组的NK细胞活性恢复至放疗前水平，且显著高于对照组（p<0.05）。3）SPG对CD4/CD8比值无显著影响。4）在我们的患者中未观察到SPG对肝肾功能有任何显著影响。SPG组和对照组放疗后鳞状细胞癌抗原（SCC）水平均迅速下降，两组间SCC水平无显著差异。因此提示SPG不抑制放疗对癌细胞的杀伤作用。基于这些发现，得出结论：SPG不仅能促进淋巴细胞尤其是T细胞的恢复，还能促进NK细胞活性的恢复。这些免疫学发现表明SPG在子宫颈癌患者放疗中的临床应用具有一定价值。
