Kim C S, Cho T K, Jinn T H
Department of Otolaryngology, College of Medicine, Seoul National University, Korea.
Otolaryngol Head Neck Surg. 1990 Dec;103(6):918-25. doi: 10.1177/019459989010300606.
The permeability of the round window membrane (RWM) was studied by using horseradish peroxidase (HRP) in the different stages of otitis media developed in guinea pigs, and it was compared with normal RWM under transmission electron microscopic examination. The experimental model of otitis media was developed by inoculation of a Pseudomonas aeruginosa suspension. When HRP could penetrate into the inner layer of a normal RWM, the duration of HRP instillation into the round window niche was 10 minutes. The permeability of the RWM in otitis media was as follows: 3-day animals showed penetration of HRP into the middle layer. In 1-week animals, HRP had penetrated into the inner layer in all 10 ears. In 2-week animals, HRP had penetrated into the inner layer in 6 of 11 ears. In 4-week animals, however, HRP was not seen in 6 of 7 ears. HRP had penetrated through the damaged focus of the epithelial cells by inflammation. These results suggest that any derangement of the epithelial linings could increase the penetration of HRP and also ototoxic materials into the inner ear through the RWM.
通过在豚鼠中耳不同阶段的中耳炎模型中使用辣根过氧化物酶（HRP）来研究圆窗膜（RWM）的通透性，并在透射电子显微镜检查下将其与正常圆窗膜进行比较。中耳炎实验模型通过接种铜绿假单胞菌悬液建立。当HRP能够穿透正常圆窗膜的内层时，向圆窗龛滴注HRP的持续时间为10分钟。中耳炎中圆窗膜的通透性如下：3日龄动物显示HRP穿透至中层。1周龄动物中，10只耳朵的HRP均已穿透至内层。2周龄动物中，11只耳朵中有6只的HRP已穿透至内层。然而，在4周龄动物中，7只耳朵中有6只未见到HRP。HRP通过炎症导致的上皮细胞损伤部位穿透。这些结果表明，上皮衬里的任何紊乱都可能增加HRP以及耳毒性物质通过圆窗膜进入内耳的穿透性。
