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非洲爪蟾卫星1 DNA的长程组织和序列导向曲率

Long-range organization and sequence-directed curvature of Xenopus laevis satellite 1 DNA.

作者信息

Pasero P, Sjakste N, Blettry C, Got C, Marilley M

机构信息

Laboratoire de Génétique, URA CNRS 1189, Faculté de Médecine, Marseille, France.

出版信息

Nucleic Acids Res. 1993 Oct 11;21(20):4703-10. doi: 10.1093/nar/21.20.4703.

DOI:10.1093/nar/21.20.4703
PMID:7901836
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC331494/
Abstract

We have investigated the long-range organization and the intrinsic curvature of satellite 1 DNA, an unusual tandemly-repeated DNA family of Xenopus laevis presenting sequence homologies to SINEs. PFGE was used in combination with frequent-cutter restriction enzymes not likely to cut within satellite 1 DNA and revealed that almost all the repeating units are tandemly organized to form large arrays (200 kb to 2 Mb) that are marked by restriction length polymorphism and contain intra-array domains of sequence variation. Besides that, we have analysed the secondary structure of satellite 1 DNA by computer modelling. Theoretical maps of curvature obtained from three independent models of DNA bending (the dinucleotide wedge model of Trifonov, the junction model of Crothers and the model of de Santis) showed that satellite 1 DNA is intrinsically curved and these results were confirmed experimentally by polyacrylamide gel electrophoresis. Moreover, we observed that this bending element is highly conserved among all the members of the satellite 1 DNA family that are accessible to analysis. A potential genetic role for satellite 1 DNA based on this unusual structural feature is discussed.

摘要

我们研究了卫星1 DNA的长程组织和固有曲率，卫星1 DNA是非洲爪蟾一个不同寻常的串联重复DNA家族，与短散在核元件（SINEs）存在序列同源性。脉冲场凝胶电泳（PFGE）与不太可能在卫星1 DNA内部切割的常用切割限制酶联合使用，结果显示几乎所有的重复单元都串联排列形成大的阵列（200 kb至2 Mb），这些阵列以限制片段长度多态性为特征，并且包含序列变异的阵列内结构域。除此之外，我们通过计算机建模分析了卫星1 DNA的二级结构。从三个独立的DNA弯曲模型（Trifonov的二核苷酸楔形模型、Crothers的连接模型以及de Santis模型）获得的曲率理论图谱表明，卫星1 DNA具有固有曲率，并且这些结果通过聚丙烯酰胺凝胶电泳得到了实验证实。此外我们观察到，在所有可用于分析的卫星1 DNA家族成员中，这种弯曲元件高度保守。基于这一不同寻常的结构特征，我们讨论了卫星1 DNA潜在的遗传作用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6a69/331494/8185645b0733/nar00069-0059-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6a69/331494/2db0b6ba1822/nar00069-0057-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6a69/331494/acc7b18c7a44/nar00069-0057-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6a69/331494/73f9a771bf2d/nar00069-0058-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6a69/331494/8185645b0733/nar00069-0059-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6a69/331494/2db0b6ba1822/nar00069-0057-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6a69/331494/acc7b18c7a44/nar00069-0057-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6a69/331494/73f9a771bf2d/nar00069-0058-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6a69/331494/8185645b0733/nar00069-0059-a.jpg

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