Department of Biochemistry and Institute for Medical Sciences, Chonbuk National University, Medical School, Jeonju, Jeonbuk 560-182, Republic of Korea.
Biochem Biophys Res Commun. 2011 Feb 25;405(4):552-7. doi: 10.1016/j.bbrc.2011.01.065. Epub 2011 Jan 22.
Dihydroavenanthramide D (DHAvD) is a synthetic analog to naturally occurring avenanthramide, which is the active component of oat. Previous study demonstrates that DHAvD strongly inhibits activation of nuclear factor-kappa B (NF-κB), which is a major component in cancer cell invasion. The present study investigated whether DHAvD can modulate MMP-9 expression and cell invasion in MCF-7 human breast cancer cells. MMP-9 expression and cell invasion in response to 12-O-tetradecanoylphorbol-13-acetate (TPA) was increased, whereas these inductions were muted by DHAvD. DHAvD also suppressed activation of mitogen-activated protein kinase (MAPK), and MAPK-mediated nuclear factor-kappa B (NF-κB) and activator protein-1 (AP-1) activations in TPA-treated MCF-7 cells. The results indicate that DHAvD-mediated inhibition of TPA-induced MMP-9 expression and cell invasion involves the suppression of the MAPK/NF-κB and MAPK/AP-1 pathways in MCF-7 cells. DHAvD may have potential value in breast cancer metastasis.
二氢燕麦酰胺 D（DHAvD）是一种天然燕麦酰胺的合成类似物，燕麦酰胺是燕麦的活性成分。先前的研究表明，DHAvD 可强烈抑制核因子-κB（NF-κB）的激活，NF-κB 是癌细胞侵袭的主要成分。本研究探讨了 DHAvD 是否可以调节 MCF-7 人乳腺癌细胞中 MMP-9 的表达和细胞侵袭。12-O-十四烷酰佛波醇-13-醋酸酯（TPA）可增加 MMP-9 的表达和细胞侵袭，而 DHAvD 可减弱这些诱导作用。DHAvD 还抑制了丝裂原活化蛋白激酶（MAPK）的激活，以及 TPA 处理的 MCF-7 细胞中 MAPK 介导线粒体核因子-κB（NF-κB）和激活蛋白-1（AP-1）的激活。结果表明，DHAvD 介导的 TPA 诱导的 MMP-9 表达和细胞侵袭抑制涉及 MCF-7 细胞中 MAPK/NF-κB 和 MAPK/AP-1 通路的抑制。DHAvD 在乳腺癌转移中可能具有潜在价值。
