Department of Nutrition and Food Science, Texas A&M University, College Station, TX 77843, USA.
Fitoterapia. 2011 Jun;82(4):557-69. doi: 10.1016/j.fitote.2011.01.013. Epub 2011 Jan 22.
Polyphenolics extracted from yaupon holly (Ilex vomitoria, Aquifoliaceae) (YH) leaves were investigated in human colon cells for their chemopreventive and anti-inflammatory activities. An activity-guided fractionation allowed the selection of YH flavonol-rich fraction due to its preferential inhibition of HT-29 colon cancer viability over the normal CCD-18Co colon cells. Quercetin and kaempferol 3-rutinosides, main components identified in this fraction, protected CCD-18Co cells against reactive oxidative species (ROS) in part due to increased activity of antioxidant enzymes. In addition, up-regulation of microRNA-146a (miR-146a) known as a negative regulator of pro-inflammatory NF-κB activation was the underlying molecular mechanism that protected CCD-18Co from inflammation.
从冬青叶（冬青科）中提取的多酚类化合物（冬青叶）被研究用于人类结肠细胞的化学预防和抗炎活性。活性导向的分级分离选择了富含 YH 类黄酮的级分，因为它优先抑制 HT-29 结肠癌细胞的活力，而不是正常的 CCD-18Co 结肠细胞。在该级分中鉴定出的主要成分槲皮素和山奈酚 3-芸香糖苷，部分通过增加抗氧化酶的活性来保护 CCD-18Co 细胞免受活性氧（ROS）的侵害。此外，上调已知作为促炎 NF-κB 激活的负调节剂的 microRNA-146a（miR-146a）是保护 CCD-18Co 免受炎症的潜在分子机制。
