干酪乳杆菌 01 细胞碎片和胞外多糖的抗增殖和抗细胞毒性作用。
Antiproliferative and anticytotoxic effects of cell fractions and exopolysaccharides from Lactobacillus casei 01.
机构信息
Graduate Institute of Food Science and Technology, National Taiwan University, Taipei 10617, Taiwan.
出版信息
Mutat Res. 2011 Apr 3;721(2):157-62. doi: 10.1016/j.mrgentox.2011.01.005. Epub 2011 Jan 22.
Cell fractions including heat-treated cells, crude cell walls, intracellular extracts and exopolysaccharides (EPSs) obtained from Lactobacillus casei 01 were first studied for their effects on the proliferation of human intestinal epithelial cells, intestine 407 and the human colon cancer cell, HT-29. Their effects on the cytotoxicity of 4-nitroquinoline 1-oxide (4-NQO) against intestine 407 were further investigated. The results revealed that EPS exhibited the highest antiproliferation activity on HT-29 cells while the viability of intestine 407 cells was not affected by EPS at a concentration of 5-50μg/mL. It was also noted that all the cell fractions and EPS from L. casei 01 reduced the cytotoxicity of 4-NQO against intestine 407 with EPS showing the highest anticytotoxic activity. Additionally, it was found that EPS might exert blocking and bioanticytotoxic effects by both adjusting the function of intestine 407 and repairing the 4-NQO-damaged cells, thus reducing cytotoxicity of 4-NQO.
从干酪乳杆菌 01 中提取的细胞碎片（包括热处理细胞、粗细胞壁、细胞内提取物和胞外多糖（EPS）），首先研究了它们对人肠道上皮细胞、肠 407 和人结肠癌细胞 HT-29 增殖的影响。进一步研究了它们对 4-硝基喹啉 1-氧化物（4-NQO）对肠 407 细胞毒性的影响。结果表明，EPS 对 HT-29 细胞的增殖抑制活性最高，而 EPS 在 5-50μg/mL 浓度下对肠 407 细胞的活力没有影响。还注意到，L. casei 01 的所有细胞片段和 EPS 均降低了 4-NQO 对肠 407 的细胞毒性，其中 EPS 显示出最高的抗细胞毒性活性。此外，发现 EPS 可能通过调节肠 407 的功能和修复 4-NQO 损伤的细胞来发挥阻断和生物抗细胞毒性作用，从而降低 4-NQO 的细胞毒性。
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