Choi S S, Kim Y, Han K S, You S, Oh S, Kim S H
Division of Food Science, Korea University, Seoul, South Korea.
Lett Appl Microbiol. 2006 May;42(5):452-8. doi: 10.1111/j.1472-765X.2006.01913.x.
The objective of this study was to assess in vitro, whether heat-killed (HK) lactic acid bacteria cells and fractionations of HK cells could suppress the viability of human cancer cells and inhibit the cytotoxicity associated with oxidative stress.
Among the strains, the HK cells of Lactobacillus acidophilus 606 and Lactobacillus casei ATCC 393 exhibited the most profound inhibitory activity in all of the tested cell lines. HK cells of L. acidophilus 606 were determined to be less toxic to healthy human embryo fibroblasts (hEF cells) than were HK cells of L. casei ATCC 393. The soluble polysaccharides from L. acidophilus 606 evidenced the most effective anticancer activity, but inhibited hEF cell growth by only 20%. The soluble polysaccharides from L. acidophilus 606 were partly observed to induce apoptosis in the HT-29 cells by DNA fragmentation and propidium iodine staining. Both the HK cells of L. acidophilus 606 and the soluble polysaccharide components of this strain also exhibited potent antioxidative activity.
Our findings suggest that the soluble polysaccharide fraction from L. acidophilus 606 may constitute a novel anticancer agent, which manifests a high degree of selectivity for human cancer cells and antioxidative agent in the food industry.
These soluble polysaccharide components from Lactobacillus may be applied to various foods, and used as adjuncts for cancer therapy and prevention.
本研究的目的是在体外评估热灭活（HK）乳酸菌细胞及其分级分离物是否能够抑制人癌细胞的活力，并抑制与氧化应激相关的细胞毒性。
在这些菌株中，嗜酸乳杆菌606和干酪乳杆菌ATCC 393的热灭活细胞在所有测试的细胞系中表现出最显著的抑制活性。嗜酸乳杆菌606的热灭活细胞对健康人胚胎成纤维细胞（hEF细胞）的毒性被确定低于干酪乳杆菌ATCC 393的热灭活细胞。嗜酸乳杆菌606的可溶性多糖表现出最有效的抗癌活性，但仅抑制hEF细胞生长20%。通过DNA片段化和碘化丙啶染色部分观察到嗜酸乳杆菌606的可溶性多糖可诱导HT - 29细胞凋亡。嗜酸乳杆菌606的热灭活细胞及其该菌株的可溶性多糖成分均还表现出强大的抗氧化活性。
我们的研究结果表明，嗜酸乳杆菌606的可溶性多糖级分可能构成一种新型抗癌剂，其对人癌细胞具有高度选择性，并且在食品工业中可作为抗氧化剂。
这些来自乳酸菌的可溶性多糖成分可应用于各种食品，并用作癌症治疗和预防的辅助剂。
