Liu Sheng, Hua Hong
3-D Visualization and Imaging Systems Laboratory, College of Optical Sciences, University of Arizona, Tucson, Arizona 85721, USA.
Opt Express. 2011 Jan 3;19(1):353-62. doi: 10.1364/OE.19.000353.
Increasing the depth-of-field (DOF) while maintaining high resolution imaging has been a classical challenge for conventional microscopes. Extended DOF (EDOF) is especially essential for imaging thick specimens. We present a microscope capable of capturing EDOF images in a single shot. A volumetric optical sampling method is applied by rapidly scanning the focus of a vari-focal microscope objective throughout the extended depths of a thick specimen within the duration of a single detector exposure. An EDOF image is reconstructed by deconvolving the captured image with the response function of the system. Design of a vari-focal objective and algorithms for restoring EDOF images are presented. Proof-of-concept experimental results demonstrate significantly extended DOF compared to the conventional microscope counterparts.
在保持高分辨率成像的同时增加景深（DOF）一直是传统显微镜面临的经典挑战。扩展景深（EDOF）对于厚标本成像尤为重要。我们展示了一种能够单次拍摄捕获EDOF图像的显微镜。通过在单个探测器曝光期间，在厚标本的扩展深度范围内快速扫描可变焦距显微镜物镜的焦点，应用了一种体积光学采样方法。通过使用系统的响应函数对捕获的图像进行去卷积来重建EDOF图像。介绍了可变焦距物镜的设计和恢复EDOF图像的算法。概念验证实验结果表明，与传统显微镜相比，景深得到了显著扩展。
