Laboratory of Food Microbiology and Leuven Food Science and Nutrition Research Centre (LFoRCe), Department of Microbial and Molecular Systems (M²S), Faculty of Bioscience Engineering, Katholieke Universiteit Leuven, Leuven, Belgium.
mBio. 2011 Jan 25;2(1):e00130-10. doi: 10.1128/mBio.00130-10.
Pressure and temperature are important environmental variables that influence living systems. However, while they vary over a considerable range on Earth and other planets, it has hardly been addressed how straightforwardly and to what extent cellular life can acquire resistance to extremes of these parameters within a defined genomic context and a limited number of generations. Nevertheless, this is a very pertinent question with respect to the penetration of life in allegedly inhospitable environments. In this study, directed evolution was used to reveal the potential of the nonsporulating and mesophilic model bacterium Escherichia coli to develop the ability to survive exposure to high temperature or pressure. While heat resistance could only marginally be increased, our data show that piezoresistance could readily and reproducibly be extended into the GPa range, thereby greatly exceeding the currently recognized maximum for growth or survival.
压力和温度是影响生命系统的重要环境变量。然而，尽管它们在地球和其他行星上的变化范围很大，但在一个确定的基因组背景和有限的几代内，细胞生命可以在多大程度上以及多么直接地获得对这些参数极端值的抗性，这几乎没有得到解决。然而，就生命在据称不适宜居住的环境中的渗透而言，这是一个非常相关的问题。在这项研究中，定向进化被用来揭示非孢子形成和中温模式细菌大肠杆菌发展承受高温或高压暴露能力的潜力。虽然耐热性只能略有提高，但我们的数据表明，压阻可以很容易且可重复地扩展到 GPa 范围，从而大大超过目前公认的生长或生存的最大值。
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