School of Mechanical Engineering, Purdue University, West Lafayette, IN 47907, USA.
Lab Chip. 2011 Mar 21;11(6):1161-5. doi: 10.1039/c0lc00512f. Epub 2011 Jan 24.
We report a method for fabricating inexpensive microfluidic platforms on paper using laser treatment. Any paper with a hydrophobic surface coating (e.g., parchment paper, wax paper, palette paper) can be used for this purpose. We were able to selectively modify the surface structure and property (hydrophobic to hydrophilic) of several such papers using a CO(2) laser. We created patterns down to a minimum feature size of 62±1 µm. The modified surface exhibited a highly porous structure which helped to trap/localize chemical and biological aqueous reagents for analysis. The treated surfaces were stable over time and were used to self-assemble arrays of aqueous droplets. Furthermore, we selectively deposited silica microparticles on patterned areas to allow lateral diffusion from one end of a channel to the other. Finally, we demonstrated the applicability of this platform to perform chemical reactions using luminol-based hemoglobin detection.
我们报告了一种使用激光处理在纸上制造廉价微流控平台的方法。任何具有疏水表面涂层的纸张（例如羊皮纸、蜡纸、调色板纸）都可以用于此目的。我们能够使用 CO2 激光选择性地修饰几种此类纸张的表面结构和性质（从疏水变为亲水）。我们创建的图案最小特征尺寸可达 62±1 µm。改性后的表面呈现出高度多孔的结构，有助于捕获/定位用于分析的化学和生物水性试剂。处理过的表面在一段时间内保持稳定，并用于自组装水性液滴阵列。此外，我们选择性地在图案化区域上沉积了硅胶微颗粒，以允许从通道的一端到另一端的横向扩散。最后，我们证明了该平台在使用基于鲁米诺的血红蛋白检测进行化学反应方面的适用性。
