日本与婴儿肉毒中毒相关的A型肉毒杆菌菌株及其毒素的独特特征。

Distinct characters of Clostridium botulinum type A strains and their toxin associated with infant botulism in Japan.

作者信息

Sakaguchi G, Sakaguchi S, Kamata Y, Tabita K, Asao T, Kozaki S

机构信息

Department of Veterinary Science, College of Agriculture, University of Osaka Prefecture, Japan.

出版信息

Int J Food Microbiol. 1990 Dec;11(3-4):231-41. doi: 10.1016/0168-1605(90)90016-x.

DOI:10.1016/0168-1605(90)90016-x

PMID:2126444

Abstract

Four strains of Clostridium botulinum type A having been associated with infant botulism in Japan, and another strain isolated from honey not associated with infant botulism, were found to be hemagglutinin (HA) negative. These strains do not produce L (Mr 500 kDa) nor LL toxin (Mr 900 kDa) but M toxin (Mr 300 kDa) only. No marked difference was found between the HA-positive and HA-negative strains in other biochemical properties, but the HA-negative strains tended to colonize more easily in the intestines of infant mice than did HA-positive strains. The toxin of HA-positive strains and that of HA-negative strains differed in the antigenicity of part of the toxic component and that of the nontoxic component, and in the molecular size of the toxic component.

摘要

在日本，已发现四株A型肉毒梭菌与婴儿肉毒中毒有关，另有一株从蜂蜜中分离出的与婴儿肉毒中毒无关的菌株，被发现血凝素（HA）呈阴性。这些菌株不产生L毒素（500 kDa）和LL毒素（900 kDa），仅产生M毒素（300 kDa）。HA阳性菌株和HA阴性菌株在其他生化特性上未发现明显差异，但HA阴性菌株比HA阳性菌株更容易在幼鼠肠道中定殖。HA阳性菌株的毒素和HA阴性菌株的毒素在部分毒性成分和无毒成分的抗原性以及毒性成分的分子大小方面存在差异。