Postgraduate Medical Institute, The University of Hull, Hull, UK.
Cell Biochem Funct. 2011 Jan-Feb;29(1):71-3. doi: 10.1002/cbf.1712. Epub 2010 Dec 27.
The mechanisms of hyperbaric oxygen (HBO) therapy have not been fully elucidated. It is presumed that breathing 100% oxygen at pressure generates free radicals and the cellular response to these may confer protection. A crucial signalling molecule, nuclear transcription factor kappa B (NFκB), translocates from the cytoplasm to the nucleus and activates a raft of pathways in response to various stimuli, and plays a role in inflammatory processes. This study focussed upon the expression of NFκB in isolated human peripheral blood mononuclear cells (PBMC) following HBO. Ten healthy male volunteers underwent a single HBO treatment, breathing 100% oxygen at a pressure of 2.8 ATA for 1 h. EDTA blood samples were taken pre-, post- and 4-h post-HBO. PBMC were isolated, nuclear extracts prepared and assayed using a NFkBp50 transcription factor ELISA. Mean NFκB expression of 0.27 (0.20-0.34, 95%CI) absorbance units (mg protein)(-1) was observed pre-HBO and this increased to 0.29 (0.20-0.38, 95%CI) immediately post-HBO. A significant increase in NFκB expression within PBMC was observed 4-h post-HBO, in comparison to pre-HBO (mean 0.38, 0.30-0.47, 95%CI, p = 0.027). This study demonstrates that HBO induces NFκB activation in human PBMC, which could be a crucial step in the mechanism of HBO treatment.
高压氧（HBO）治疗的机制尚未完全阐明。据推测，在压力下呼吸 100%的氧气会产生自由基，而细胞对此的反应可能提供保护。一种关键的信号分子，核转录因子 kappa B（NFκB），从细胞质转移到细胞核，并响应各种刺激激活一系列途径，在炎症过程中发挥作用。这项研究集中在 HBO 后分离的人外周血单核细胞（PBMC）中 NFκB 的表达。10 名健康男性志愿者接受了单次 HBO 治疗，在 2.8ATA 的压力下呼吸 100%的氧气 1 小时。在 HBO 之前、之后和之后 4 小时采集 EDTA 血液样本。分离 PBMC，制备核提取物，并使用 NFkBp50 转录因子 ELISA 进行检测。观察到 HBO 前的 NFκB 表达平均为 0.27（0.20-0.34，95%CI）吸光度单位（mg 蛋白）（-1），立即 HBO 后增加到 0.29（0.20-0.38，95%CI）。与 HBO 前相比，在 HBO 后 4 小时观察到 PBMC 中的 NFκB 表达显著增加（平均 0.38，0.30-0.47，95%CI，p=0.027）。这项研究表明，HBO 诱导人 PBMC 中 NFκB 的激活，这可能是 HBO 治疗机制中的关键步骤。
