Department of Sport, Health and Exercise Science, University of Hull, Hull, UK.
Cell Stress Chaperones. 2011 May;16(3):339-43. doi: 10.1007/s12192-010-0246-2. Epub 2010 Dec 5.
Hyperbaric oxygen (HBO) is thought to confer protection to cells via a cellular response to free radicals. This process may involve increased expression of heat shock proteins, in particular the highly inducible heat shock protein 72 (Hsp72). Healthy male volunteers (n = 16) were subjected to HBO for 1 h at 2.8 ATA. Inducible Hsp72 expression was measured by flow cytometry pre-, post- and 4 h-post HBO. Peripheral blood mononuclear cells (PBMC) were isolated from whole blood via density centrifugation pre-, post- and 4 h post-HBO. PBMC were then subjected to an in vitro heat shock at 40°C or hypoxia at 37°C (5% O(2)) with a control at 37°C. Cells were then analysed for Hsp72 expression by flow cytometry. Monocytes showed no significant changes in Hsp72 expression following HBO. No detectable Hsp72 was seen in lymphocytes or neutrophils. Following in vitro hypoxic exposure, a significant increase in Hsp72 expression was observed in monocytes isolated immediately post- (p = 0.006) and 4 h post-HBO (p = 0.010) in comparison to control values. HBO does not induce Hsp72 expression in PBMC. The reported benefits of HBO in terms of pre-conditioning are not due to inducement of Hsp72 expression in circulating blood cells, but may involve an enhancement of the stress response.
高压氧（HBO）被认为通过细胞对自由基的反应来保护细胞。这个过程可能涉及热休克蛋白表达的增加，特别是高度诱导的热休克蛋白 72（Hsp72）。16 名健康男性志愿者接受了 2.8ATA 下 1 小时的 HBO。通过流式细胞术在 HBO 之前、之后和 4 小时后测量诱导型 Hsp72 表达。通过密度离心从全血中分离外周血单核细胞（PBMC），在 HBO 之前、之后和 4 小时后进行。然后将 PBMC 置于体外热休克（40°C）或缺氧（37°C，5%O2）下，以 37°C 为对照。通过流式细胞术分析细胞中 Hsp72 的表达。HBO 后单核细胞中 Hsp72 表达无明显变化。淋巴细胞或中性粒细胞中未检测到 Hsp72。在体外缺氧暴露后，立即在 HBO 后（p=0.006）和 4 小时后（p=0.010）分离的单核细胞中观察到 Hsp72 表达显著增加，与对照值相比。HBO 不会诱导 PBMC 中的 Hsp72 表达。HBO 在预处理方面的报道益处不是由于循环血细胞中 Hsp72 表达的诱导，而是可能涉及应激反应的增强。
