蜡样芽孢杆菌在玻璃纸膜上的质粒转化
Plasmid transformation of Bacillus cereus on cellophane membranes.
作者信息
Sabelnikov A G, Ulyashova L V
机构信息
All Union Research Institute of Protein Biosynthesis, Moscow, U.S.S.R.
出版信息
FEMS Microbiol Lett. 1990 Oct;60(1-2):123-6. doi: 10.1016/0378-1097(90)90357-v.
A simple approach to test the ability of bacteria to undergo natural genetic transformation is suggested. The basic feature of the approach is the cultivation of bacterial cells in the presence of exogenous (plasmid) DNA on cellophane membranes placed successively on nutrient and selective agar. Using this approach the ability of Bacillus cereus for "natural" genetic transformation was detected. Transformation frequencies varied from 10(-8) to 10(-6).
本文提出了一种简单的方法来测试细菌进行自然遗传转化的能力。该方法的基本特点是将细菌细胞在含有外源（质粒）DNA的情况下，在依次放置于营养琼脂和选择性琼脂上的玻璃纸膜上进行培养。利用这种方法检测到了蜡样芽孢杆菌的“自然”遗传转化能力。转化频率在10^(-8)到10^(-6)之间。
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