质粒pWBG637从金黄色葡萄球菌到表皮葡萄球菌和粪肠球菌的接合转移。
Conjugal transfer of plasmid pWBG637 from Staphylococcus aureus to Staphylococcus epidermidis and Streptococcus faecalis.
作者信息
Udo E E, Grubb W B
机构信息
School of Medical Technology, Curtin University of Technology, Perth, Australia.
出版信息
FEMS Microbiol Lett. 1990 Oct;60(1-2):183-7. doi: 10.1016/0378-1097(90)90369-2.
Plasmids pWBG636 (GmR) and pWBG642 (EmR) derived from the conjugative plasmid pWBG637 were tested for ability to transfer conjugatively to Staphylococcus epidermidis, Streptococcus faecalis, Bacillus subtilis and Escherichia coli. Both plasmids transferred to S. epidermidis and Strept. faecalis but not to B. subtilis and E. coli. Once in S. epidermis and Strept. faecalis they were transferred back to S. aureus. Restriction endonuclease analysis showed that the plasmids were stably maintained in S epidermidis and Strept. faecalis.
对源自接合质粒pWBG637的质粒pWBG636（庆大霉素抗性）和pWBG642（红霉素抗性）进行了测试，以检测它们向表皮葡萄球菌、粪肠球菌、枯草芽孢杆菌和大肠杆菌进行接合转移的能力。两种质粒都能转移至表皮葡萄球菌和粪肠球菌，但不能转移至枯草芽孢杆菌和大肠杆菌。一旦进入表皮葡萄球菌和粪肠球菌，它们又能转移回金黄色葡萄球菌。限制性内切酶分析表明，这些质粒在表皮葡萄球菌和粪肠球菌中能稳定维持。
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