Krcméry V, Langsádl L, Antal M, Seckárová A
J Hyg Epidemiol Microbiol Immunol. 1985;29(2):141-6.
Three strains belonging to gramnegative non-fermenting rods, i.e. a Pseudomonas maltophilia strain and two strains of Acinetobacter, were tested, as representatives of different types of nosocomial strains, for transferability of their multiple drug resistance. As all of them posed difficulties in demonstrating the transferability of their resistance by conventional methods, a three-step procedure was developed that includes a transfer to rifampicin-resistant P. aeruginosa recipients, then to susceptible P. aeruginosa intermediate strains, and, finally, from these strains to rifampicin-resistant Enterobacteriaceae. In three strains studied three genetically different types of R plasmids have been demonstrated. P. maltophilia transferred Amikacin resistance, as well as resistance to other antibiotics, to P. aeruginosa and then to Enterobacteria. In contrast, an Amikacin-resistant Acinetobacter with quite identical multiple drug resistance spectrum transferred its resistance to P. aeruginosa only, but not to Enterobacteria. Finally, another Acinetobacter strain, resistant to Gentamicin but susceptible to Amikacin transferred this resistance directly to Enterobacteria (and, separately, to P. aeruginosa, too). All three strains transferred Cefamandole resistance together with other resistances. Non-fermenting rods, thus, might be a source of transmissible resistance to reserve antibiotics as Amikacin, and advanced-type Cephalosporins.
测试了三种革兰氏阴性非发酵菌，即一株嗜麦芽窄食单胞菌和两株不动杆菌，作为不同类型医院菌株的代表，检测其多重耐药性的可转移性。由于用传统方法证明它们耐药性的可转移性均存在困难，因此开发了一种三步程序，包括将耐药性转移至耐利福平的铜绿假单胞菌受体，然后转移至敏感的铜绿假单胞菌中间菌株，最后从这些菌株转移至耐利福平的肠杆菌科细菌。在所研究的三株菌中，已证明存在三种基因不同类型的R质粒。嗜麦芽窄食单胞菌将阿米卡星耐药性以及对其他抗生素的耐药性转移给了铜绿假单胞菌，然后又转移给了肠杆菌。相比之下，一株具有完全相同多重耐药谱的耐阿米卡星不动杆菌仅将其耐药性转移给了铜绿假单胞菌，而未转移给肠杆菌。最后，另一株对庆大霉素耐药但对阿米卡星敏感的不动杆菌菌株将这种耐药性直接转移给了肠杆菌（也分别转移给了铜绿假单胞菌）。所有三株菌均将头孢孟多耐药性与其他耐药性一起转移。因此，非发酵菌可能是对阿米卡星等储备抗生素和新型头孢菌素产生可传播耐药性的来源。
