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[运用电子顺磁共振方法对放线菌冻干过程中所用保护介质的有效性进行比较评估]

[Use of the electron paramagnetic resonance method for the comparative evaluation of the effectiveness of the protective media used in the lyophilization of actimomycetes].

作者信息

Kuznetsov V D, Filippova S N, Ruuge E K

出版信息

Mikrobiologiia. 1978 Jul-Aug;47(4):706-10.

PMID:212671
Abstract

The accumulation of free radicals correlates with an increase in the percentage of killed lyophilized spores of actinomycetes. Therefore, the EPR method in combination with the method of "accelerated storage" at 37 degrees C was used to evaluate rapidly (during one month) the effectiveness and to select certain protective media for stabilization of actinomycetes cultures. The EPR method can be applied for comparative evaluation of only those media which did not contain originally free radicals.

摘要

自由基的积累与放线菌冻干孢子死亡率的增加相关。因此,采用电子顺磁共振(EPR)方法结合在37℃下的“加速储存”方法,以便快速(在一个月内)评估有效性,并选择某些保护培养基来稳定放线菌培养物。EPR方法仅可用于对那些原本不含自由基的培养基进行比较评估。

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