Semenov S M
Antibiotiki. 1977 Aug;22(8):722-6.
Viability, cultural features and antibiotic-production properties of the organisms producing tetracycline, chlortetracycline, erythromycin, neomycin, oxytetracycline and polymyxin were studied after their storage for 2 years in ampoules at a temperature of 4--10 degrees in lyophilized state with the use of sodium glutamate, polyvinylpyrrolidone, their combination and horse serum. The highest growth rate was observed in most of the cultures lyophilized in sodium glutamate. The growth of the cultures lyophilized in the solution of polyvinylpyrrolidone alone was mainly scanty or moderate. The antibiotic production level in some strains lyophilized in sodium glutamate or its combination with polyvinylpyrrolidone was after storage for 2 years somewhat higher than that in the control. The cultural features, i.e. the colour of the aerial and substrate mycelium and pigment secretion did not significantly differ in the lyophilized cultures and the cultures maintained on agarized media.
对生产四环素、金霉素、红霉素、新霉素、土霉素和多粘菌素的微生物，在冻干状态下于4 - 10摄氏度的温度下在安瓿中保存2年后，使用谷氨酸钠、聚乙烯吡咯烷酮、它们的组合以及马血清，研究了这些微生物的活力、培养特性和抗生素生产特性。在大多数用谷氨酸钠冻干的培养物中观察到最高的生长速率。单独用聚乙烯吡咯烷酮溶液冻干的培养物的生长主要稀疏或适中。在谷氨酸钠或其与聚乙烯吡咯烷酮的组合中冻干的一些菌株，储存2年后的抗生素生产水平比对照略高。冻干培养物与在琼脂培养基上保存的培养物的培养特性，即气生菌丝和基内菌丝的颜色以及色素分泌，没有显著差异。
