Kuznetsov V D, Filippova S N, Murav'eva S A, Fishman V M
Mikrobiologiia. 1977 Mar-Apr;46(2):318-23.
A technique of "accelerated storage" was used to evaluate promptly the effectiveness of protective (suspension) media for lyophilization and further storage of spores of Actinomyces parvullus 99. The number of survived spores can be predicted using the Arrhenius diagram and the van't Hoff rule. Equine serum without a conserving agent was found to be the most effective protective medium for lyophilizing spores of Act. parvullus, as compared to such media as milk containing no fat or a mixture of 3 per cent solutions of gelatin and glucose.
采用“加速储存”技术迅速评估保护（悬浮）介质对微小放线菌99号孢子冻干及后续储存的有效性。存活孢子的数量可通过阿累尼乌斯图和范特霍夫规则进行预测。与不含脂肪的牛奶或明胶和葡萄糖3%溶液混合物等介质相比，不含防腐剂的马血清被发现是冻干微小放线菌孢子最有效的保护介质。
