Tiergesundheitsdienst Bayern e.V. , Senator-Gerauer-Strasse 23, 85586 Poing, Germany.
J Agric Food Chem. 2011 Feb 23;59(4):1423-7. doi: 10.1021/jf103751z. Epub 2011 Jan 26.
The aim of this study was to collect further data about levels of endogenous hormones in foodstuffs derived from animal production. Because of expected higher concentrations of especially estrogens in colostrum compared to other foodstuffs, our investigation focused on this matrix. For evaluation of endogenous steroid hormones in separated colostrum (fat and defatted fraction) and colostrum powder, the relevant free and conjugated estrogens (estradiol-17β, estradiol-17α, estrone, and estriol) androgens (androstendione, 19nor-androstendione, 19nor-testosterone-17β, 19nor-testosterone-17α, testosterone-17β, and testosterone-17α), and progesterone were determined by means of liquid chromatography-tandem mass spectrometry (LC-MS/MS). Upmost determined concentrations were found in the fat fraction, with 25.56 and 7.59 μg/L for estrone and androstendione, respectively. In defatted milk and colostrum powder, conjugated estrogens dominated, whereas total (free and conjugated) estrone (5.51 μg/L; 15.0 μg/kg) exceeded estradiol-17α (2.66 μg/L; 7.5 μg/kg) and estradiol-17β (2.28 μg/L; 3.3 μg/kg). Neither 19nor-steroids nor estriol were detected in colostrum fractions or processed colostrum.
本研究的目的是收集更多关于动物生产衍生食品中内源性激素水平的数据。由于初乳中尤其是雌激素的浓度预计会高于其他食品，因此我们的调查重点放在了这个基质上。为了评估分离的初乳（脂肪和脱脂部分）和初乳粉中的内源性甾体激素，相关的游离和共轭雌激素（雌二醇-17β、雌二醇-17α、雌酮和雌三醇）、雄激素（雄烯二酮、19 去甲雄烯二酮、19 去甲睾酮-17β、19 去甲睾酮-17α、睾酮-17β 和睾酮-17α）和孕激素通过液相色谱-串联质谱法（LC-MS/MS）进行了测定。在脂肪部分中，雌酮和雄烯二酮的浓度最高，分别为 25.56 和 7.59μg/L。在脱脂奶和初乳粉中，共轭雌激素占主导地位，而总（游离和共轭）雌酮（5.51μg/L；15.0μg/kg）超过雌二醇-17α（2.66μg/L；7.5μg/kg）和雌二醇-17β（2.28μg/L；3.3μg/kg）。初乳部分和加工后的初乳中均未检测到 19 去甲甾体和雌三醇。
