Guo Xiaoyu, Gong Huili, Zhao Wenji
Beijing Key Lab for Resources Environment and Geography Information System, Capital Normal University, Beijing 100037, China.
Wei Sheng Wu Xue Bao. 2010 Nov;50(11):1519-24.
In order to obtain the information about how irrigations with recycled water affecting the cultivable microbial population of the rhizosphere of turfgrasses.
We isolated strains from two irrigated areas with recycled water (RW) and drinking water (DW) in Taoranting Park in Beijing, China, by using diluting plate and counting method, and analyzing the 16S rDNA sequence of the isolates.
We obtained 20 and 25 strains with different morphological character of colonies from the area irrigated with DW and area irrigated with RW, respectively. The sequence analysis of 16SrDNA showed that RW-irrigated system supported more complex communities of 18 genus and 24 species, whereas the DW-irrigated system only supported 15 genus and 20 species. Both samples had similar microbial population. There were 9.7% or 13.4% alphaproteobacteria, 8.1% or 12.3% betaproteobacteria, 17.9% or 42.0% gammaproteobacteria, 13.0% or 2.9% bacteroidetes, 23.6% or 10.1% firmicutes, and 27.6% or 19.6% actinobacteria at the rhizosphere of turfgrasses irrigated with DW or RW respectively. The dominant bacteria in DW area were the genera Bacillus whereas that of RW area was the genera Acinetobacter. Other than the gammaproteobacteria, the dominant genera in other groups of two areas were similar.
The result showed that the bacterial community composition of rhizosphere samples did not change, but the distribution of different types of bacteria does change after irrigation by reclaimed water, which were determined by the abundance increasing of the dominant species and the appearance or disappearance of non-dominant species. Finally, it is important that the control of the pathogen and metal should be enforced when using RW for irrigation.
为了获取有关再生水灌溉如何影响草坪草根际可培养微生物种群的信息。
我们采用稀释平板计数法，从中国北京陶然亭公园两个分别用再生水（RW）和饮用水（DW）灌溉的区域分离菌株，并分析分离株的16S rDNA序列。
我们分别从用DW灌溉的区域和用RW灌溉的区域获得了20株和25株具有不同菌落形态特征的菌株。16SrDNA序列分析表明，用RW灌溉的系统支持更复杂的群落，有18属24种，而用DW灌溉的系统仅支持15属20种。两个样本的微生物种群相似。在分别用DW或RW灌溉的草坪草根际，α-变形菌分别占9.7%或13.4%，β-变形菌分别占8.1%或12.3%，γ-变形菌分别占17.9%或42.0%，拟杆菌分别占13.0%或2.9%，厚壁菌分别占23.6%或10.1%，放线菌分别占27.6%或19.6%。DW区域的优势细菌是芽孢杆菌属，而RW区域的是不动杆菌属。除γ-变形菌外，两个区域其他菌群中的优势属相似。
结果表明，根际样本的细菌群落组成没有变化，但再生水灌溉后不同类型细菌的分布发生了变化，这是由优势物种丰度的增加以及非优势物种的出现或消失所决定的。最后，使用RW进行灌溉时，对病原体和金属的控制至关重要。
