Cui Bing-Jian, Gao Feng, Hu Chao, Li Zhong-Yang, Fan Xiang-Yang, Cui Er-Ping
Farmland Irrigation Research Institute, Chinese Academy of Agricultural Sciences, Xinxiang 453002, China.
Key Laboratory of High-efficient and Safe Utilization of Agriculture Water Resources, Chinese Academy of Agricultural Sciences, Xinxiang 453002, China.
Huan Jing Ke Xue. 2019 Nov 8;40(11):5151-5163. doi: 10.13227/j.hjkx.201904269.
Reclaimed water is considered to be a reasonable and sustainable alternative water resource to improve water resource layout and mitigate the shortage of traditional water resources. Its use in irrigation will cause changes in the microbial community structure and opportunistic pathogen abundance in soils and crops, but few studies have been conducted on this subject. Peppers were used as the research subjects, and the treatments were direct irrigation of reclaimed water, mixed irrigation with freshwater and reclaimed water, rotated irrigation with freshwater and reclaimed water, with potable water irrigation as the control. The effects of different irrigation methods of reclaimed water on the soil physicochemical properties were analyzed through a pot experiment. Furthermore, changes in bacterial community and opportunistic pathogen abundance in pepper fruit and the rhizosphere under reclaimed water irrigation conditions were investigated based on high-throughput sequencing technology and quantitative PCR methods. The results showed that direct irrigation with reclaimed water increased soil EC and decreased soil pH. 16S rDNA high-throughput sequencing showed that Proteobacteria, Bacteroides, Actinobacteria, and Firmicutes were present in both pepper fruit and the rhizosphere at phylum level, and the most dominant genera (, , , , , and ) were greatly affected by reclaimed water irrigation methods. Quantitative PCR results indicated that the influence of reclaimed water irrigation on the distribution and abundance of pathogenic bacteria in the soil-pepper system was different, and the abundance of spp. in pepper fruit and in the rhizosphere increased with reclaimed water irrigation. Our results indicated that the reclaimed water was suitable for agricultural irrigation, but different reclaimed water irrigation methods may introduce different degrees of microbial contamination. In addition, attention must be given to some opportunistic pathogens and phytopathogens.
再生水被认为是一种合理且可持续的替代水资源，有助于改善水资源布局并缓解传统水资源短缺问题。其用于灌溉会导致土壤和作物中微生物群落结构及机会性病原体丰度发生变化，但针对这一主题的研究较少。以辣椒作为研究对象，处理方式包括直接灌溉再生水、淡水与再生水混合灌溉、淡水与再生水交替灌溉，以饮用水灌溉作为对照。通过盆栽试验分析了再生水不同灌溉方式对土壤理化性质的影响。此外，基于高通量测序技术和定量PCR方法，研究了再生水灌溉条件下辣椒果实和根际细菌群落及机会性病原体丰度的变化。结果表明，直接灌溉再生水会增加土壤电导率并降低土壤pH值。16S rDNA高通量测序显示，辣椒果实和根际在门水平上均存在变形菌门、拟杆菌门、放线菌门和厚壁菌门，且最主要的属（……）受再生水灌溉方式影响较大。定量PCR结果表明，再生水灌溉对土壤 - 辣椒系统中病原菌的分布和丰度影响不同，再生水灌溉使辣椒果实中的……菌和根际中的……菌丰度增加。我们的结果表明，再生水适合农业灌溉，但不同的再生水灌溉方式可能会引入不同程度的微生物污染。此外，必须关注一些机会性病原体和植物病原体。
