Department of Biology, Cork Institute of Technology, Cork, Ireland.
Vet Microbiol. 2011 May 5;149(3-4):482-7. doi: 10.1016/j.vetmic.2010.12.001. Epub 2010 Dec 8.
The aim of this study was to characterize 38 bovine strains of Mycobacterium avium subsp. paratuberculosis isolated in Ireland using 11 multi locus short sequence repeat (MLSSR) loci and 8 mycobacterial interspersed repetitive units-variable number of tandem repeat (MIRU-VNTR) loci. The discriminatory power of these two typing methods alone and combined was evaluated, as was the epidemiology of the isolates and the genotypes obtained. Using the MIRU-VNTR typing method (8 loci analysed), only 3 subtypes were detected with a discrimination index (DI) of 0.54, but one MIRU-VNTR type has not been identified in other studies. In contrast the MLSSR method (using 11 loci) differentiated the 38 MAP isolates into 18 types with DI of 0.92. Among these 18 types 6 have not been recorded previously. The combination of the 2 methods (MIRU-VNTR and MLSSR) produced 22 distinct genotypes giving a maximal DI of 0.94. According to the allelic diversity, some markers are more polymorphic than others and must be applied in priority for the differentiation of MAP bovine isolates. This is the first report of genotyping data for MAP on the island of Ireland and will be very useful for analysing its epidemiology, transmission and virulence.
本研究旨在使用 11 个多位点短重复序列（MLSSR）和 8 个分枝杆菌插入重复可变数串联重复（MIRU-VNTR）位点，对在爱尔兰分离的 38 株牛分枝杆菌副结核亚种进行鉴定。评估了这两种单独和组合的分型方法的区分能力，以及分离株的流行病学和获得的基因型。使用 MIRU-VNTR 分型方法（分析 8 个位点），仅检测到 3 种亚型，区分指数（DI）为 0.54，但有一种 MIRU-VNTR 类型在其他研究中未被识别。相比之下，MLSSR 方法（使用 11 个位点）将 38 株 MAP 分离株分为 18 种类型，DI 为 0.92。在这 18 种类型中，有 6 种以前没有记录过。两种方法（MIRU-VNTR 和 MLSSR）的组合产生了 22 种不同的基因型，最大 DI 为 0.94。根据等位基因多样性，一些标记比其他标记更具多态性，必须优先应用于 MAP 牛分离株的分化。这是爱尔兰岛 MAP 基因分型数据的首次报告，将非常有助于分析其流行病学、传播和毒力。
