Centro de Vigilancia Sanitaria Veterinaria VISAVET, Universidad Complutense de Madrid, Avda Puerta de Hierro, 28040 Madrid, Spain.
Vet Microbiol. 2010 Jul 29;144(1-2):118-26. doi: 10.1016/j.vetmic.2009.12.028. Epub 2010 Jan 4.
Mycobacterial interspersed repetitive units and variable number tandem repeats typing (MIRU-VNTR) is a useful technique that has been recently applied to characterize members of the Mycobacterium avium complex (MAC). The aim of this study was to examine the genetic variability among a collection of Spanish M. avium subspecies paratuberculosis (M. a. paratuberculosis) isolates with a combination of MIRU-VNTR loci. For this purpose we tested six MIRU-VNTR loci (MIRU-2, MIRU-3, VNTR-25, VNTR-32, VNTR-292 and VNTR-259) in 70 M. a. paratuberculosis isolates of Types II and III that were recovered from 22 Spanish localities during a nine-year period (1998-2007). The combination of five loci (MIRU-2, MIRU-3, VNTR-25, VNTR-32 and VNTR-259) enabled the differentiation of 12 allelic profiles, with a resulting Hunter and Gaston discriminatory index (HGDI) of 0.84. Moreover, we obtained MIRU-VNTR patterns that were unique for each of the M. a. paratuberculosis types analyzed (II and III); other patterns were host-related or restricted to geographic areas. Therefore, this MIRU-VNTR approach could be a useful sub-typing molecular tool in order to get a better sense of the epidemiology of Johne's disease.
分枝杆菌散在重复单位和可变数串联重复序列分型（MIRU-VNTR）是一种最近被应用于描述禽分枝杆菌复合体（MAC）成员的有用技术。本研究的目的是通过组合使用 MIRU-VNTR 基因座来检测一组西班牙分枝杆菌副结核病亚种（M. a. paratuberculosis）分离株的遗传变异性。为此，我们在 70 株来自 22 个西班牙地区的 II 型和 III 型 M. a. paratuberculosis 分离株中测试了 6 个 MIRU-VNTR 基因座（MIRU-2、MIRU-3、VNTR-25、VNTR-32、VNTR-292 和 VNTR-259），这些分离株是在 9 年期间（1998-2007 年）从西班牙各地回收的。组合使用五个基因座（MIRU-2、MIRU-3、VNTR-25、VNTR-32 和 VNTR-259）可以区分 12 种等位基因谱，产生的 Hunter 和 Gaston 区分指数（HGDI）为 0.84。此外，我们获得了每种分析的分枝杆菌副结核病亚种（II 型和 III 型）所特有的 MIRU-VNTR 图谱；其他图谱与宿主相关或仅限于特定地理区域。因此，这种 MIRU-VNTR 方法可能是一种有用的亚分型分子工具，可以更好地了解约翰氏病的流行病学。
