Yildiz Hamit, Durmus Ali Said, Simsek Halil, Yaman Ihsan
Department of Obstetrics and Gynecology, Firat University, Elazig, Turkey.
Acta Cir Bras. 2011 Feb;26(1):51-7. doi: 10.1590/s0102-86502011000100010.
To compare the effects of vitamin E and 1% methylen blue solutions on prevention of experimentally induced adhesions in rats.
Thirty seven female Spraque Dawley rats were randomized into four groups. First group was kept as sham operated group. An adhesion model was constituted on the left uterine horn of the other groups. The lesion areas of rats from the second, the third and the fourth groups were coated with 2 ml 0.9 % saline solution (C group), 10 mg vitamin E (VE group) and 1% methylen blue solutions (MB group), respectively.
Histopathologically, adhesion scores, mononuclear cell infiltration, oedema and fibrosis were more prominent in the MB group compared with C and VE groups. There were no significant differences between the groups in tissue glutathione peroxidase (GPx), catalase (CAT) activities and glutation (GSH) level, these parameters were slightly increased in group with VE supplementation though. The administration of VE and MB significantly decreased NO (P<0.01) levels when compared to the C group. The level of malondialdehyde (MDA) in the VE group was significantly lower (P<0.05) than those of the Sh and C groups.
Intraperitoneal methylen blue solutions treatments were more effective according to vitamin E in preventing the formation of intra-abdominal adhesion in a rat uterine horn model.
比较维生素E和1%亚甲蓝溶液对预防大鼠实验性诱导粘连的效果。
将37只雌性斯普拉格-道利大鼠随机分为四组。第一组作为假手术组。在其他组大鼠的左子宫角构建粘连模型。分别给第二、第三和第四组大鼠的损伤部位涂抹2 ml 0.9%生理盐水溶液（C组）、10 mg维生素E（VE组）和1%亚甲蓝溶液（MB组）。
组织病理学检查显示，与C组和VE组相比，MB组的粘连评分、单核细胞浸润、水肿和纤维化更为明显。各组之间组织谷胱甘肽过氧化物酶（GPx）、过氧化氢酶（CAT）活性和谷胱甘肽（GSH）水平无显著差异，不过补充VE的组中这些参数略有升高。与C组相比，VE和MB的给药显著降低了NO（P<0.01）水平。VE组丙二醛（MDA）水平显著低于假手术组（Sh组）和C组（P<0.05）。
在大鼠子宫角模型中，腹腔注射亚甲蓝溶液在预防腹腔内粘连形成方面比维生素E更有效。
