Department of Applied Physics, Royal Institute of Technology, Stockholm, Sweden.
BMC Neurosci. 2011 Jan 27;12:16. doi: 10.1186/1471-2202-12-16.
The Na+,K+-ATPase plays an important role for ion homeostasis in virtually all mammalian cells, including neurons. Despite this, there is as yet little known about the isoform specific distribution in neurons.
With help of superresolving stimulated emission depletion microscopy the spatial distribution of Na+,K+-ATPase in dendritic spines of cultured striatum neurons have been dissected. The found compartmentalized distribution provides a strong evidence for the confinement of neuronal Na+,K+-ATPase (α3 isoform) in the postsynaptic region of the spine.
A compartmentalized distribution may have implications for the generation of local sodium gradients within the spine and for the structural and functional interaction between the sodium pump and other synaptic proteins. Superresolution microscopy has thus opened up a new perspective to elucidate the nature of the physiological function, regulation and signaling role of Na+,K+-ATPase from its topological distribution in dendritic spines.
Na + ，K + -ATP 酶在包括神经元在内的几乎所有哺乳动物细胞的离子动态平衡中起着重要作用。尽管如此，目前对于神经元中同工型的特异性分布知之甚少。
借助超分辨率受激发射损耗显微镜，解析了培养纹状体神经元树突棘中 Na + ，K + -ATP 酶的空间分布。发现的分区分布为神经元 Na + ，K + -ATP 酶（α 3 同工型）局限在棘突的突触后区域提供了有力证据。
分区分布可能对棘突内局部钠离子梯度的产生以及钠泵与其他突触蛋白之间的结构和功能相互作用具有重要意义。因此，超分辨率显微镜为阐明 Na + ，K + -ATP 酶的生理功能、调节和信号作用的本质提供了一个新的视角，从其在树突棘中的拓扑分布来看。
