Herbal Medicine EBM Research Center, Korea Institute of Oriental Medicine, Exporo 483, Yusung-gu, Daejeon 305-811, Republic of Korea.
Food Chem Toxicol. 2011 May;49(5):1047-55. doi: 10.1016/j.fct.2011.01.010. Epub 2011 Jan 25.
In this study, we analyzed the anti-inflammatory effects of Angelica dahurica Bentham et Hooker ethanolic extract (ADEE) on RAW264.7 cells, to understand the mechanism underlying its observed effects. ADEE inhibited cyclooxygenase 2 (COX-2) and inducible nitric oxide synthase (iNOS) expression, leading to the suppression of COX-2-derived prostaglandin E(2) and iNOS-derived production in lipopolysaccharide (LPS)-stimulated RAW264.7 cells. These inhibitory effects of ADEE were accompanied by the reduced production of tumor necrosis factor α and interleukin (IL)-6. ADEE also inhibited nuclear factor κB (NFκB) translocation to the nucleus by interrupting inhibitor kappa Bα (IκBα) degradation. ADEE upregulated heme oxygenase 1 expression, and treatment with tin protoporphyrin IX (SnPP), a selective inhibitor of HO-1, reversed the LPS-induced generation of proinflammatory cytokines. ADEE also induced IL-4 and IL-5 expression in concanavalin-A-stimulated splenocytes. These results suggest that ADEE has anti-inflammatory activity, which acts via the suppression of the NF-κB pathway.
在这项研究中，我们分析了白芷乙醇提取物（ADEE）对 RAW264.7 细胞的抗炎作用，以了解其观察到的作用机制。ADEE 抑制环氧化酶 2（COX-2）和诱导型一氧化氮合酶（iNOS）的表达，导致 COX-2 衍生的前列腺素 E（2）和 LPS 刺激的 RAW264.7 细胞中 iNOS 衍生产物的产生受到抑制。ADEE 的这些抑制作用伴随着肿瘤坏死因子 α和白细胞介素（IL）-6 的产生减少。ADEE 通过中断抑制剂 kappa Bα（IκBα）降解来抑制核因子 κB（NFκB）向核内易位。ADEE 上调血红素加氧酶 1 的表达，并用血红素加氧酶 1 的选择性抑制剂锡原卟啉 IX（SnPP）处理可逆转 LPS 诱导的促炎细胞因子的产生。ADEE 还在刀豆球蛋白 A 刺激的脾细胞中诱导白细胞介素 4 和白细胞介素 5 的表达。这些结果表明，ADEE 具有抗炎活性，通过抑制 NF-κB 途径发挥作用。
