Food Science and Technology College, Hua Zhong Agriculture University, Wuhan, Hubei, PR China.
Spectrochim Acta A Mol Biomol Spectrosc. 2011 Apr;78(4):1266-71. doi: 10.1016/j.saa.2010.12.074. Epub 2010 Dec 30.
A new method for determination of lysozyme with high sensitivity based on Resonance Rayleigh scattering (RRS) by using Au nanoparticles as a probe was proposed in this experiment. The RRS spectrum, nonlinear scattering (second-order scattering (SOS), frequency doubling scattering (FDS)) spectra and absorption spectrum of the Au nanoparticles-lysozyme system have been analyzed. In addition, the effects of several factors on scattering intensities were investigated, including pH value of solution, amount of Au nanoparticles, mixing sequence of each reagent and the coexisting substances. The results showed that the coexisting substances have little influence on the RRS intensities of the systems. Moreover, the possible mechanism for the RRS enhancement of Au nanoparticles-lysozyme system was preliminary discussed. The RRS method for determination of lysozyme has good sensitivity and selectivity with the detection limits 30.1 ng/ml. The contents of lysozyme were determined with recoveries of 98.2-105.4% and relativity relative standard deviation (RSD) of 0.3-3.7%, respectively. It proved that the method established in our study is suitable for the determination of lysozyme in synthetic sample and natural chicken egg white sample.
本实验提出了一种基于金纳米粒子作为探针的高灵敏度溶菌酶测定新方法，用于共振瑞利散射（RRS）。分析了金纳米粒子-溶菌酶体系的 RRS 光谱、非线性散射（二阶散射（SOS）、倍频散射（FDS））光谱和吸收光谱。此外，还研究了几种因素对散射强度的影响，包括溶液的 pH 值、金纳米粒子的用量、各试剂的混合顺序以及共存物质。结果表明，共存物质对体系的 RRS 强度影响不大。此外，还初步探讨了金纳米粒子-溶菌酶体系 RRS 增强的可能机制。该 RRS 法测定溶菌酶具有良好的灵敏度和选择性，检测限为 30.1ng/ml。用该方法测定合成样品和天然鸡蛋白样品中的溶菌酶含量，回收率为 98.2%-105.4%，相对标准偏差（RSD）为 0.3%-3.7%。结果表明，本研究建立的方法适用于合成样品和天然鸡蛋白样品中溶菌酶的测定。
