SalGI限制性核酸内切酶。酶的特异性。
The SalGI restriction endonuclease. Enzyme specificity.
作者信息
Maxwell A, Halford S E
出版信息
Biochem J. 1982 Apr 1;203(1):93-8. doi: 10.1042/bj2030093.
Abstract
We have analysed the kinetics of DNA cleavage in the reaction between the SalGI restriction endonuclease and plasmid pMB9. This reaction is subject to competitive inhibition by DNA sequences outside the SalGI recognition site; we have determined the Km and Vmax. for the reaction of this enzyme at its recognition site and the KI for its interaction at other DNA sequences. We conclude that the specificity of DNA cleavage by the enzyme is only partly determined by the discrimination it shows for binding at its recognition sequence compared with binding to other DNA sequences.
摘要
我们分析了SalGI限制性内切核酸酶与质粒pMB9之间反应中DNA切割的动力学。该反应受到SalGI识别位点以外的DNA序列的竞争性抑制；我们已经确定了该酶在其识别位点反应的Km和Vmax，以及它在其他DNA序列处相互作用的KI。我们得出结论，该酶切割DNA的特异性仅部分取决于它在识别序列处结合与在其他DNA序列处结合相比所表现出的区分能力。
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本文引用的文献
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2
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3
The EcoRI restriction endonuclease, covalently closed DNA and ethidium bromide.EcoRI 限制性内切酶、共价闭合 DNA 和溴化乙锭。
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The EcoRI restriction endonuclease with bacteriophage lambda DNA. Equilibrium binding studies.
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