Pulst S M, Korenberg J R
Division of Neurology, Cedars-Sinai Medical Center, University of California, Los Angeles 90048.
Am J Med Genet Suppl. 1990;7:137-40. doi: 10.1002/ajmg.1320370728.
Some forms of familial Alzheimer disease (FAD) have shown linkage to DNA probes for the loci D21S1 and D21S16 on chromosome 21. To investigate the physical location of these DNA probes, we have constructed a physical map of this region of chromosome 21 by using quantitative Southern blot analysis of DNA sequences unique to chromosome 21 and a series of cell lines aneuploid for parts of chromosome 21. We now show that both D21S16 and D21S1/S11 are located in the same region of chromosome 21, in q11.2-q21.05. This new panel of aneuploid cell lines will allow the rapid mapping of new DNA probes in the vicinity of the FAD gene.
某些形式的家族性阿尔茨海默病（FAD）已显示与21号染色体上D21S1和D21S16位点的DNA探针存在连锁关系。为了研究这些DNA探针的物理位置，我们利用对21号染色体特有的DNA序列进行定量Southern印迹分析以及一系列部分21号染色体非整倍体的细胞系，构建了21号染色体该区域的物理图谱。我们现在表明，D21S16和D21S1/S11都位于21号染色体的同一区域，即q11.2 - q21.05。这个新的非整倍体细胞系面板将有助于在FAD基因附近快速定位新的DNA探针。
