Padmanabhan S, Han J Y, Nanayankkara I, Tran K, Ho P, Mesfin N, White I, DeVoe D L
Department of Chemical and Biomolecular Engineering, University of Maryland, College Park, Maryland 20742, USA.
Department of Mechanical Engineering, University of Maryland, College Park, Maryland 20742, USA.
Biomicrofluidics. 2020 Feb 18;14(1):014113. doi: 10.1063/1.5126938. eCollection 2020 Jan.
Sample filling and discretization within thermoplastic 2D microwell arrays is investigated toward the development of low cost disposable microfluidics for passive sample discretization. By using a high level of contact angle asymmetry between the filling channel and microwell surfaces, a significant increase in the range of well geometries that can be successfully filled is revealed. The performance of various array designs is characterized numerically and experimentally to assess the impact of contact angle asymmetry and device geometry on sample filling and discretization, resulting in guidelines to ensure robust microwell filling and sample isolation over a wide range of well dimensions. Using the developed design rules, reliable and bubble-free sample filling and discretization is achieved in designs with critical dimensions ranging from 20 m to 800 m. The resulting devices are demonstrated for discretized nucleic acid amplification by performing loop-mediated isothermal amplification for the detection of the mecA gene associated with methicillin-resistant .
为了开发用于被动样品离散化的低成本一次性微流控技术，对热塑性二维微孔阵列中的样品填充和离散化进行了研究。通过在填充通道和微孔表面之间使用高度的接触角不对称性，发现可成功填充的孔几何形状范围显著增加。对各种阵列设计的性能进行了数值和实验表征，以评估接触角不对称性和器件几何形状对样品填充和离散化的影响，从而得出确保在广泛的孔尺寸范围内实现稳健的微孔填充和样品分离的指导原则。利用所制定的设计规则，在关键尺寸范围为20μm至800μm的设计中实现了可靠且无气泡的样品填充和离散化。通过对与耐甲氧西林相关的mecA基因进行环介导等温扩增检测，展示了所得器件用于离散核酸扩增的能力。
