来自电鳐富含乙酰胆碱受体的膜中的固定化脂质。
Immobilized lipid in acetylcholine receptor-rich membranes from Torpedo marmorata.
作者信息
Marsh D, Barrantes F J
出版信息
Proc Natl Acad Sci U S A. 1978 Sep;75(9):4329-33. doi: 10.1073/pnas.75.9.4329.
Abstract
The lipid environment of acetylcholine receptor-rich membranes from Torpedo marmorata has been studied with spin labels. The electron spin resonance spectra of both stearic acid and steroid probes in the membranes revealed an immobilized lipid component, in addition to the fluid component which is found in aqueous bilayer dispersions of the extracted lipids. The spin labels also cause a differential paramagnetic quenching of the intrinsic protein fluorescence of the membranes, which is sensitive to the action of cholinergic ligands and follows a modified Stern-Volmer law. Electron spin resonance difference spectroscopy shows that the protein-associated lipid is immobilized with respect to rotation both around and perpendicular to the long molecular axis, with correlation times : formula: (see text) approximately 50-70 ns. The proportion of lipid in the immobilized component is greater than calculated for a single boundary layer around the protein and corresponds more closely to the total interstitial lipid occupying the area between densely packed protein units in acetylcholine receptor-rich membranes.
摘要
用自旋标记法研究了电鳐富含乙酰胆碱受体的膜的脂质环境。膜中硬脂酸和类固醇探针的电子自旋共振光谱显示，除了在提取脂质的水双层分散体中发现的流体成分外，还存在固定化脂质成分。自旋标记还会导致膜固有蛋白质荧光的差异顺磁猝灭，这对胆碱能配体的作用敏感，并遵循修正的斯特恩-沃尔默定律。电子自旋共振差异光谱表明，与蛋白质相关的脂质在围绕分子长轴以及垂直于分子长轴的旋转方面都是固定化的，相关时间约为50 - 70纳秒。固定化成分中脂质的比例大于针对蛋白质周围单个边界层计算的比例，并且更接近于占据富含乙酰胆碱受体的膜中紧密堆积的蛋白质单元之间区域的总间隙脂质。
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