Johnson-Muller B, Gross J
Proc Natl Acad Sci U S A. 1978 Sep;75(9):4417-21. doi: 10.1073/pnas.75.9.4417.
Mixtures of epithelial and stromal cells isolated from normal adult rabbit cornea, when cocultured in the presence of cytochalasin B, produced latent collagenase, whereas neither cell type alone, nor the mixture in the absence of this agent, did so. The enzyme, a characteristic animal collagenase, required proteolytic activation. The relative concentrations of epithelial and stromal cells had a profound effect on on collagenase production, the enzyme activity bieng directly proportional to the number of stromal cells but inversely proportional to the number of epithelial cells. The amount of enzyme released into the medium was also directly proportional to cytochalasin B concentration. Media conditioned by cytochalasin B-treated epithelial or stromal cells did not stimulate collagenase secretion by the other cell type. The data suggest direct cell contact or close proximity as the mode of productive interaction and tentatively identify the stromal cell as the source of enzyme and the epithelial cell as a stimulator.
从正常成年兔角膜分离出的上皮细胞和基质细胞混合物，在细胞松弛素B存在的情况下共培养时，会产生潜在的胶原酶，而单独的任何一种细胞类型，或不存在该试剂的混合物都不会产生这种酶。这种酶是一种典型的动物胶原酶，需要蛋白水解激活。上皮细胞和基质细胞的相对浓度对胶原酶的产生有深远影响，酶活性与基质细胞数量成正比，但与上皮细胞数量成反比。释放到培养基中的酶量也与细胞松弛素B浓度成正比。经细胞松弛素B处理的上皮细胞或基质细胞条件培养基不会刺激另一种细胞类型分泌胶原酶。数据表明直接细胞接触或紧密接近是产生相互作用的方式，并初步确定基质细胞为酶的来源，上皮细胞为刺激物。
