Johnson-Wint B, Gross J
J Cell Biol. 1984 Jan;98(1):90-6. doi: 10.1083/jcb.98.1.90.
We have examined the ability of primary adult rabbit skin cells to regulate collagenase production in vitro. Dermal cells constitutively produce collagenase in culture, and enzyme production by these cells can be influenced by epithelial cells. Co-culture with skin epidermal cells resulted in more enzyme production by dermal cells, whereas co-culture with corneal epithelial cells yielded less enzyme activity. Connective tissue cells from a different source, cornea, also produced collagenase when co-cultured with skin epidermal cells, although the stromal cells alone made no enzyme. The drug cytochalasin B had very little influence on collagenase production by dermal cells, either alone or in co-culture with epidermal cells, but did significantly potentiate enzyme production by corneal stromal cells responding to epidermal effector molecules. Epidermal-cell-conditioned medium from both fetal and adult rabbit skin was a potent source of stimulators (apparent mol wt 20,500 and 55,000) of connective-tissue-cell collagenase production. Stimulator production by epidermal cultures was cell density dependent. Optimal production of stimulators occurred in adult cultures containing 10(6) epidermal cells/ml of medium, and in fetal cultures containing 10(5) cells/ml. Inhibitors of connective tissue cell enzyme production were not detected in conditioned medium from either adult or fetal epidermal cells.
我们研究了成年兔原代皮肤细胞在体外调节胶原酶产生的能力。真皮细胞在培养过程中组成性地产生胶原酶，这些细胞产生的酶可受上皮细胞影响。与皮肤表皮细胞共培养会使真皮细胞产生更多的酶，而与角膜上皮细胞共培养则产生较少的酶活性。来自不同来源（角膜）的结缔组织细胞在与皮肤表皮细胞共培养时也会产生胶原酶，尽管单独的基质细胞不产生酶。药物细胞松弛素B对真皮细胞单独或与表皮细胞共培养时胶原酶的产生影响很小，但能显著增强角膜基质细胞对表皮效应分子反应时的酶产生。来自胎儿和成年兔皮肤的表皮细胞条件培养基是结缔组织细胞胶原酶产生的刺激物（表观分子量分别为20,500和55,000）的有效来源。表皮培养物中刺激物的产生取决于细胞密度。刺激物的最佳产生发生在每毫升培养基含10⁶个表皮细胞的成年培养物以及每毫升含10⁵个细胞的胎儿培养物中。在成年或胎儿表皮细胞的条件培养基中未检测到结缔组织细胞酶产生的抑制剂。
