Mohammadi Rahim, Azizi Saeed, Delirezh Nowruz, Hobbenaghi Rahim, Amini Keyvan
Department of Clinical Sciences, Faculty of Veterinary Medicine, Urmia University, Nazloo Road, Urmia, Iran.
Chin J Traumatol. 2011 Feb 1;14(1):46-52.
Present study aimed at further comprehensive functional, histomorphometrical and immunohistochemical assessment of peripheral nerve regeneration using rat sciatic nerve transection model.
The 10-mm rat sciatic nerve gap was created in rats. In control group nerve stumps were sutured to adjacent muscle and in treatment group the gap was bridged using an inside-out vein graft. In sham-operated group the nerve was manipulated and left intact. All animals underwent walking track analysis test 4, 8, and 12 weeks after surgery. Subsequently, muscle mass measurement was performed to assess reenervation, histological examination to observe the sciatic nerve regeneration morphologically and immunohistochemistry to detect Schwann cells using anti S-100. Results were analyzed using a factorial ANOVA with two between-subjects factors. Bonferroni test for pairwise comparisons was used to examine the effect of treatments.
Functional analysis of myelinated nerve fibers showed that nerve function improved significantly in the time course in treatment group. However, quantitative morphometrical analysis of myelinated nerve fibers showed that there was no significant difference between 8 and 12 weeks in treatment group. Muscle weight ratio was bigger and weight loss of the gastrocnemius muscle was ameliorated by inside-out vein grafting. The position of positive immunohistochemical reactions further implied that regenerated axons and Schwann cell-like cells existed after vein grafting was performed, and was accompanied by the process of myelination and structural recovery of regenerated nerves.
Functional analysis of peripheral nerve repair is far more reliable than quantitative morphometrical analysis.
本研究旨在利用大鼠坐骨神经横断模型，对外周神经再生进行进一步全面的功能、组织形态计量学和免疫组织化学评估。
在大鼠中制造10毫米的坐骨神经缺损。对照组将神经残端缝合至相邻肌肉，治疗组使用外翻静脉移植物桥接缺损。假手术组对神经进行操作但保持完整。所有动物在术后4、8和12周进行行走轨迹分析测试。随后，进行肌肉质量测量以评估再支配情况，进行组织学检查以形态学观察坐骨神经再生，并用抗S-100免疫组织化学检测雪旺细胞。结果采用含两个组间因素的析因方差分析进行分析。采用Bonferroni检验进行两两比较以检验治疗效果。
有髓神经纤维的功能分析表明，治疗组在时间进程中神经功能显著改善。然而，有髓神经纤维的定量形态计量学分析表明，治疗组在8周和12周之间无显著差异。外翻静脉移植使肌肉重量比增大，腓肠肌重量减轻得到改善。免疫组织化学阳性反应的位置进一步表明，静脉移植后存在再生轴突和雪旺细胞样细胞，并伴有再生神经的髓鞘形成和结构恢复过程。
外周神经修复的功能分析比定量形态计量学分析可靠得多。
