Neurophysiology Research Center, Department of Anatomy, Faculty of Medicine, Urmia University of Medical Sciences, Urmia, Iran.
Iran J Basic Med Sci. 2013 Aug;16(8):901-5.
OBJECTIVE(S): The aim of this study was to evaluate the final outcome of nerve regeneration across the eggsell membrane (ESM) tube conduit in comparison with autograft.
Thirty adult male rats (250-300 g) were randomized into (1) ESM conduit, (2) autograft, and (3) sham surgery groups. The eggs submerged in 5% acetic acid. The decalcifying membranes were cut into four pieces, rotated over the teflon mandrel and dried at 37(°)C. The left sciatic nerve was surgically cut. A 10-mm nerve segment was cut and removed. In the ESM group, the proximal and distal cut ends of the sciatic nerve were telescoped into the nerve guides. In the autograft group, the 10 mm nerve segment was reversed and used as an autologous nerve graft. All animals were evaluated by sciatic functional index (SFI) and electrophysiology testing.
The improvement in SFI from the first to the last evalution in ESM and autograft groups were evaluated. On days 49 and 60 post-operation, the mean SFI of ESM group was significantly greater than the autograft group (P< 0.05). On day 90, the mean nerve conduction velocity (NCV) of ESM group was greater than autograft group, although the difference was not statistically significant (P> 0.05).
These findings demonstrate that ESM effectively enhances nerve regeneration and promotes functional recovery in injured sciatic nerve of rat.
本研究旨在评估鸡蛋膜（ESM）管导管与自体移植物在神经再生方面的最终结果。
将 30 只成年雄性大鼠（250-300g）随机分为（1）ESM 导管组、（2）自体移植物组和（3）假手术组。将鸡蛋浸泡在 5%的醋酸中。脱钙膜被切成四块，在特氟龙芯棒上旋转并在 37°C 下干燥。将左侧坐骨神经进行手术切割。切除 10mm 的神经节段。在 ESM 组中，将坐骨神经的近侧和远侧切割端插入神经导管中。在自体移植物组中，将 10mm 的神经节段反转并用作自体神经移植物。所有动物均通过坐骨神经功能指数（SFI）和电生理学测试进行评估。
评估了 ESM 和自体移植物组从第一次到最后一次评估的 SFI 改善情况。在术后第 49 和 60 天，ESM 组的平均 SFI 明显大于自体移植物组（P<0.05）。在第 90 天，ESM 组的平均神经传导速度（NCV）大于自体移植物组，尽管差异无统计学意义（P>0.05）。
这些发现表明，ESM 有效地增强了大鼠受损坐骨神经的神经再生和功能恢复。
