Department of Experimental Veterinary Sciences, Università degli Studi di Padova, Agripolis Viale dell'Università 16, Legnaro (PD), Italy.
Aquat Toxicol. 2011 Feb;101(3-4):512-20. doi: 10.1016/j.aquatox.2010.12.003. Epub 2010 Dec 13.
Several studies carried out in the last years have demonstrated the presence of a wide range of contaminants in some areas of the Venice Lagoon. Many of these contaminants are able to drive free radical reactions, which lead to oxidative stress and can potentially affect fish health. In the present study, oxidative stress biomarkers were examined in three different sites (Porto Marghera, Val di Brenta and Caroman) of the Venice Lagoon and their levels monitored in Zosterisessor ophiocephalus, one of the most common fish species present in the lagoon. Schmorl's staining revealed the presence of melanomacrophage centres in spleen and head kidney, and the highest number of melanomacrophage centres was observed in the animals sampled at the Porto Marghera (Porto Marghera vs Val di brenta and Caroman: p<0.01). The cellular localization of HNE and NT, investigated through an immunohistochemical approach, showed that immunopositivity was mainly localized in melanomacrophage centres of spleen and kidney. It is relevant that the animals of the detoxified control group did not exhibit any immunoreactivity. By Western blot, the antibodies against HNE and NT recognized in the liver polypeptides damaged by oxidative stress with molecular weights under 66kDa. Comparing the relative densities, animals from the Val di Brenta site exhibited the lowest levels of HNE adducts (p<0.05), whereas animals from the Porto Marghera site exhibited the highest levels of NT adducts (p<0.05). MDA levels, measured spectrophotometrically by TBARS assay did not exhibit any statistical difference among sites.
近年来的多项研究表明，威尼斯泻湖的一些区域存在着广泛的污染物。其中许多污染物能够引发自由基反应，导致氧化应激，并可能对鱼类健康产生影响。在本研究中，对威尼斯泻湖的三个不同地点（玛格丽塔港、瓦尔迪布伦塔和卡罗曼）的氧化应激生物标志物进行了检测，并监测了其水平在黄颡鱼（Zosterisessor ophiocephalus）中的变化，黄颡鱼是该泻湖中最常见的鱼类之一。Schmorl 染色显示，脾脏和头肾中存在黑色素巨噬细胞中心，在玛格丽塔港（Porto Marghera）采样的动物中观察到的黑色素巨噬细胞中心数量最多（Porto Marghera 与 Val di Brenta 和 Caroman 相比：p<0.01）。通过免疫组织化学方法研究 HNE 和 NT 的细胞定位，发现免疫阳性主要定位于脾脏和肾脏的黑色素巨噬细胞中心。值得注意的是，解毒对照组的动物没有表现出任何免疫反应性。通过 Western blot，针对 HNE 和 NT 的抗体在肝多肽中识别出了氧化应激损伤的产物，分子量在 66kDa 以下。比较相对密度，来自瓦尔迪布伦塔（Val di Brenta）地点的动物表现出最低水平的 HNE 加合物（p<0.05），而来自玛格丽塔港（Porto Marghera）地点的动物表现出最高水平的 NT 加合物（p<0.05）。通过 TBARS 测定法分光光度法测量的 MDA 水平在各地点之间没有显示出任何统计学差异。
