Bortoletti Martina, Fonsatti Elisa, Leva Federico, Maccatrozzo Lisa, Ballarin Cristina, Radaelli Giuseppe, Caberlotto Stefano, Bertotto Daniela
Department of Comparative Biomedicine and Food Science (BCA), University of Padova, Viale dell'Università 16, I-35020 Legnaro, Padova, Italy.
Valle Cà Zuliani Società Agricola Srl, Via Pila 48, I-45018 Pila di Porto Tolle, Rovigo, Italy.
Animals (Basel). 2023 Oct 21;13(20):3288. doi: 10.3390/ani13203288.
In aquaculture, the transportation of live fish is a crucial but stress-inducing practice, necessitating a thorough understanding of its impact on fish welfare. This study aimed to assess the physiological stress response of meagre () juveniles during a 24 h commercial transport by quantifying muscle cortisol levels using a specific radioimmunoassay. Additionally, an immunohistochemical approach was used to detect and localize the cellular distribution of oxidative-stress-related biomarkers within various tissues and organs. The results demonstrated a significant increase in muscle cortisol levels following the loading procedure, remaining elevated above basal levels throughout the 24 h transport period. This effect may be attributed to either insufficient time for recovery from the loading stress or prolonged transportation-related stress. Immunostaining for all the antibodies we examined was observed in multiple tissues and organs, but we found no notable variations among the various transport phases. In conclusion, the observed stress response appears to be mainly linked to loading stress and the transport process itself, emphasizing the importance of implementing appropriate operational procedures to safeguard fish well-being during transport. Nonetheless, the unaltered distribution of oxidative stress markers between the control and transported groups suggests that the experienced stress might be within tolerable limits.
在水产养殖中，活鱼运输是一项至关重要但会引发应激的操作，因此有必要深入了解其对鱼类福利的影响。本研究旨在通过使用特定的放射免疫分析法量化肌肉皮质醇水平，评估尖吻鲈幼鱼在24小时商业运输过程中的生理应激反应。此外，采用免疫组织化学方法检测和定位氧化应激相关生物标志物在各种组织和器官内的细胞分布。结果表明，装鱼过程后肌肉皮质醇水平显著升高，在整个24小时运输期间一直高于基础水平。这种效应可能归因于从装鱼应激中恢复的时间不足或与运输相关的应激时间延长。我们检测的所有抗体的免疫染色在多个组织和器官中均有观察到，但在不同运输阶段未发现明显差异。总之，观察到的应激反应似乎主要与装鱼应激和运输过程本身有关，强调了实施适当操作程序以保障运输过程中鱼类健康的重要性。尽管如此，对照组和运输组之间氧化应激标志物分布未变，表明所经历的应激可能在可容忍范围内。
