Carminato Antonio, Pascoli Francesco, Trocino Angela, Locatello Lisa, Maccatrozzo Lisa, Palazzi Renato, Radaelli Giuseppe, Ballarin Cristina, Bortoletti Martina, Bertotto Daniela
Italian Health Authority and Research Organization for Animal Health and Food Safety, Viale dell'Università 14, 35020 Padova, Italy.
Department of Comparative Biomedicine and Food Science (BCA), University of Padova, Viale dell'Università 16, 35020 Padova, Italy.
Animals (Basel). 2020 Jul 18;10(7):1226. doi: 10.3390/ani10071226.
In the present study European sea bass () subjected to two different diets (organic vs. conventional) were evaluated in terms of growing performances, oxidative stress, and contaminant markers. Growing performances were evaluated using biometric measures and condition factor (K), whereas insulin-like growth factor (IGF-I and IGF-II) levels were assessed trough Real-Time PCR analysis. For oxidative stress, immunohistochemical staining for 8-hydroxy-2'-deoxyguanosine (8-OHdG) and 4-hydroxy-2-nonenal (HNE) was performed, whereas total glutathione (GSH) in blood serum was determined by an enzymatic method adapted. Cytochrome P4501A (CYP1A) and melanomacrophage centers (MMCs) were evaluated as contaminant markers trough immunohistochemical and histochemical approaches, respectively. The growing performances showed a positive trend in both groups but a greater productivity in conventional fed fish compared to the organic ones. A significant higher expression of MMCs was observed in organic vs. conventional diet fed fish. Fillet analysis showed a higher MUFA content and a lower PUFAs n-6 content in organically fed sea bass indicating that diets with a content in fatty acids closer to that of wild fish will definitely affect the fatty acid profile of the fish flesh. On the other hand, the diet composition did not seem to affect neither the oxidative stress parameters (GSH, 8-OHdG, HNE) nor the CYP1A expression.
在本研究中，对接受两种不同饲料（有机饲料与传统饲料）的欧洲海鲈（）的生长性能、氧化应激和污染物标志物进行了评估。使用生物测量指标和肥满度系数（K）评估生长性能，而通过实时聚合酶链反应分析评估胰岛素样生长因子（IGF-I和IGF-II）水平。对于氧化应激，进行了8-羟基-2'-脱氧鸟苷（8-OHdG）和4-羟基-2-壬烯醛（HNE）的免疫组织化学染色，而血清中的总谷胱甘肽（GSH）则通过改良的酶法测定。分别通过免疫组织化学和组织化学方法将细胞色素P4501A（CYP1A）和黑素巨噬细胞中心（MMCs）评估为污染物标志物。两组的生长性能均呈上升趋势，但与有机饲料喂养的鱼相比，传统饲料喂养的鱼生产力更高。在有机饲料与传统饲料喂养的鱼中，观察到MMCs的表达显著更高。鱼片分析表明，有机饲料喂养的海鲈中MUFA含量较高，PUFAs n-6含量较低，这表明脂肪酸含量更接近野生鱼的饲料肯定会影响鱼肉的脂肪酸谱。另一方面，饲料组成似乎既不影响氧化应激参数（GSH、8-OHdG、HNE），也不影响CYP1A的表达。
