Caetano Tânia, Krawczyk Joanna M, Mösker Eva, Süssmuth Roderich D, Mendo Sónia
Department of Biology and CESAM, University of Aveiro, 3810 Aveiro, Portugal; Medinfar-Pharmaceutical Products SA, Amadora, 2700 Venda Nova, Portugal.
Chem Biol. 2011 Jan 28;18(1):90-100. doi: 10.1016/j.chembiol.2010.11.010.
Lichenicidin is a class II two-component lantibiotic produced by Bacillus licheniformis. It is composed of the two peptides Bliα and Bliβ, which act synergistically against various Gram-positive bacteria. The lichenicidin gene cluster was successfully expressed in Escherichia coli, thus constituting the first report to our knowledge of a full reconstitution of a lantibiotic biosynthetic pathway in vivo by a Gram-negative host. This system was further exploited to characterize and assign the function of proteins encoded in the biosynthetic gene cluster in the maturation of lichenicidin peptides. Moreover, a trans complementation system was developed for expression of Bliα and Bliβ variants in vivo. This contribution will spur future studies in the heterologous expression and engineering of lantibiotics.
地衣芽胞杆菌素是由地衣芽孢杆菌产生的II类双组分羊毛硫抗生素。它由两种肽Bliα和Bliβ组成，这两种肽协同作用对抗各种革兰氏阳性菌。地衣芽胞杆菌素基因簇在大肠杆菌中成功表达，据我们所知，这是首次关于革兰氏阴性宿主在体内完全重建羊毛硫抗生素生物合成途径的报道。该系统被进一步用于表征和确定生物合成基因簇中编码的蛋白质在地衣芽胞杆菌素肽成熟过程中的功能。此外，还开发了一种反式互补系统用于在体内表达Bliα和Bliβ变体。这一成果将推动未来关于羊毛硫抗生素异源表达和工程改造的研究。
