前列环素I2对颗粒细胞环磷酸腺苷生成的刺激作用。

Prostaglandin I2 stimulation of granulosa cell cyclic AMP production.

作者信息

Goff A K, Zamecnik J, Ali M, Armstrong D T

出版信息

Prostaglandins. 1978 May;15(5):875-9. doi: 10.1016/0090-6980(78)90154-5.

Abstract

The ability of prostaglandin I2 (PGI2) to stimulate cyclic AMP production by granulosa cells, isolated from intact immature rats, has been demonstrated in vitro. The minimal effective dose was 15 ng/ml, which was comparable to the minimal effective dose for PGE2. However, a concentration of 15 microgram/ml PGI2 was required to stimulate cyclic AMP production maximally, compared to a concentration of 1 microgram/ml PGE2, which produced the maximum response. It therefore appears that PGI2 is not more effective than PGE2 in stimulating cyclic AMP production in granulosa cells, and is possibly less effective. Submaximal concentrations of PGI2 appeared to be able to modify the stimulation of cyclic AMP production by follicle-stimulating hormone (FSH), but whether or not PGI2 plays any role in follicular function remains to be established.

摘要

已在体外证实，前列腺素I2（PGI2）能刺激从完整未成熟大鼠分离出的颗粒细胞产生环磷酸腺苷（cAMP）。最小有效剂量为15 ng/ml，这与前列腺素E2（PGE2）的最小有效剂量相当。然而，刺激cAMP产生达到最大值时，PGI2需要15 μg/ml的浓度，相比之下，PGE2只需1 μg/ml的浓度就能产生最大反应。因此，在刺激颗粒细胞产生cAMP方面，PGI2似乎并不比PGE2更有效，甚至可能效果更差。低于最大剂量的PGI2似乎能够改变促卵泡激素（FSH）对cAMP产生的刺激作用，但PGI2在卵泡功能中是否发挥任何作用仍有待确定。