Garbi C, Colletta G, Cirafici A M, Marchisio P C, Nitsch L
Dipartimento di Biologia e Patologia Cellulare e Molecolare, Seconda Facoltà di Medicina, Napoli, Italy.
Eur J Cell Biol. 1990 Dec;53(2):281-9.
The action of transforming growth factor-beta (TGF-beta) on the morphology, cytoskeleton and extracellular matrix was investigated in FRTL-5 thyroid epithelial cells. After treatment with TGF-beta, FRTL-5 cells became flat and developed straight and thick bundles of actin microfilaments. This effect of TGF-beta was observed even in the presence of thyrotropin, which has a strong microfilament disrupting action. TGF-beta also influenced some aspects of the extracellular matrix organization. Immunofluorescence staining of FRTL-5 cells revealed both the appearance of a fibrillar array of fibronectin in association with the basal plasma membrane and a change in the morphology of basally located laminin patches. TGF-beta induced the formation of adhesion structures at the ventral portion of the cell membrane. Vinculin was focally concentrated at the end of stress fibers in areas corresponding to focal adhesions as revealed by interference reflection microscopy (IRM). The ability to modulate cytoskeleton organization and extracellular matrix protein distribution might mediate some of the reported TGF-beta effects on the expression of specific functional properties in thyroid cells.
在FRTL-5甲状腺上皮细胞中研究了转化生长因子-β（TGF-β）对细胞形态、细胞骨架和细胞外基质的作用。用TGF-β处理后，FRTL-5细胞变扁平，并形成笔直且粗壮的肌动蛋白微丝束。即使在存在具有强烈微丝破坏作用的促甲状腺激素的情况下，也能观察到TGF-β的这种作用。TGF-β还影响细胞外基质组织的某些方面。FRTL-5细胞的免疫荧光染色显示，与基底质膜相关的纤连蛋白纤维状阵列出现，并且基底定位的层粘连蛋白斑块的形态发生变化。TGF-β诱导细胞膜腹侧部分形成黏附结构。如干涉反射显微镜（IRM）所示，纽蛋白在对应于黏着斑的应力纤维末端局部集中。调节细胞骨架组织和细胞外基质蛋白分布的能力可能介导了一些报道的TGF-β对甲状腺细胞特定功能特性表达的影响。
