Key Lab of Multi-Organ Transplantation, Department of Hepatobiliary and Pancreatic Surgery, The First Affiliated Hospital, School of Medicine, Zhejiang University, 79# Qingchun Road, Hangzhou, Zhejiang Province 310003, China.
Med Oncol. 2012 Jun;29(2):899-908. doi: 10.1007/s12032-011-9833-z. Epub 2011 Jan 30.
This study aimed to analyze the expression of CD45 on HBV-specific T cells in patients who underwent liver transplantation (LT). We detected the distribution of two CD45 isoforms (CD45RA, CD45RO) and CD27 on peripheral blood lymphocytes including two HBV-specific pentamer cells by flow cytometry. Meanwhile, the levels of tacrolimus, plasma IL-7, and IL-15 were tested by ELISA. CD45RA decreased sharply after LT, whereas the expression of CD45RO(+) changed a very little. The proportion of CD4(+) CD27(+) subsets increased in the span from first 6-36 months after LT. With the up-regulation of IL-7, CD45RA(+) CD27(+) was reduced and CD45RO(+) was increased on HBV-specific T cells in LT group compared with CHB. Therefore, the switch from CD45RA to CD45RO probably depended on IL-7, without being stimulated by hepatitis B virus (HBV) and proliferated by IL-15. IL-7 could aid in maintaining HBV-specific T cells effectively during 3 years.
本研究旨在分析肝移植（LT）患者乙型肝炎病毒（HBV）特异性 T 细胞上 CD45 的表达。我们通过流式细胞术检测了外周血淋巴细胞中两种 CD45 同型体（CD45RA、CD45RO）和 CD27 的分布，包括两种 HBV 特异性五聚体细胞。同时，通过 ELISA 检测了他克莫司、血浆 IL-7 和 IL-15 的水平。LT 后 CD45RA 急剧下降，而 CD45RO(+) 的表达变化很小。LT 后 6-36 个月，CD4(+) CD27(+)亚群的比例增加。随着 IL-7 的上调，LT 组 HBV 特异性 T 细胞上 CD45RA(+) CD27(+)减少，CD45RO(+)增加，与 CHB 相比。因此，CD45RA 向 CD45RO 的转换可能依赖于 IL-7，而不受乙型肝炎病毒（HBV）的刺激，也不受 IL-15 的增殖。IL-7 可以在 3 年内有效帮助维持 HBV 特异性 T 细胞。
