Department of Periodontology, Operative and Preventive Dentistry, University of Bonn, Bonn, Germany.
Cancer Invest. 2011 Mar;29(3):196-201. doi: 10.3109/07357907.2010.543210. Epub 2011 Jan 31.
The objective of this study was to investigate the impact of human beta-defensins (hBDs) on oral squamous cell carcinoma (OSCC) proliferation and hBD expression in vitro. BHY-OSCC cell lines were stimulated with hBD-1, -2, and -3. Proliferation of BHY cells was ascertained and hBD-mRNA expression was evaluated by real-time PCR. Proliferation of BHY cells decreased by 25% in response to hBD-1 stimulation but increased after stimulation with hBD-2 and -3. HBD-1 stimulation enhanced hBD-3 expression, whereas HBD-2 stimulation decreased early hBD-3 expression. HBD-3 stimulation enhanced hBD-1 expression. HBDs profoundly impact on OSCC proliferation and hBD expression in vitro. Therefore, hBD-1 might function as a tumor suppressor gene in OSCCs, while hBD-2 and -3 might be protooncogenes.
本研究旨在探讨人β防御素（hBDs）对口腔鳞状细胞癌（OSCC）增殖的影响及其在体外的 hBD 表达。用 hBD-1、-2 和 -3 刺激 BHY-OSCC 细胞系。通过实时 PCR 确定 BHY 细胞的增殖和 hBD-mRNA 表达。hBD-1 刺激使 BHY 细胞增殖减少 25%，但 hBD-2 和 -3 刺激后增殖增加。hBD-1 刺激增强 hBD-3 表达，而 hBD-2 刺激早期 hBD-3 表达减少。hBD-3 刺激增强 hBD-1 表达。hBDs 对 OSCC 增殖和 hBD 表达具有深远影响。因此，hBD-1 可能在 OSCC 中作为抑癌基因发挥作用，而 hBD-2 和 -3 可能是原癌基因。
