Department of Dermatology, Shijingshan teaching hospital of Capital Medical University, Beijing Shijingshan Hospital, Beijing, China.
Department of Dermatology, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, Shanghai, China.
Skin Res Technol. 2023 Jan;29(1):e13255. doi: 10.1111/srt.13255.
To study the expression of interleukin-1β (IL-1β), interleukin-4 (IL-4), interferon-γ (IFN-γ) and tumour necrosis factor α (TNF-α) in different tissue in a dinitrochlorobenzene (DNCB)-induced ear swelling test in mice and further evaluate the correlation between the cytokine expression in different tissues and the degree of ear swelling.
The mice were sensitised with a 0.50% DNCB solution on their back for 3 days. After 7 days, the thickness of their ears was measured and grouped. Different concentrations of the DNCB solution were challenged in the left ear of each group of mice, and the right ear was used as the control. The thickness of both ears was measured every 24 h, and the mice were sacrificed 72 h after the challenge. The expressions of IL-1β, IL-4, IFN-γ and TNF-α in the mouse serum, lymph node and ear tissue were quantified by enzyme-linked immunosorbent assay, respectively.
There was a linear positive correlation between the swelling index of the mouse lateral ear and the challenge concentration of DNCB (r = 0.96, p < 0.01). The high expression of IL-1β and IL-4 in the lateral ear tissue of the mice was positively correlated with the ear swelling index 48 h after the challenge. The correlation coefficient was 0.78 (p < 0.01). Furthermore, IFN-γ and TNF-α had no significant correlation with the ear swelling index 48 h after the challenge.
There is a correlation between the degree of ear swelling in mice and the concentration of DNCB and the expression of IL-1β and IL-4 in the lateral ear tissue. There is a sub-clinical skin sensitivity state in contact allergy.
研究白细胞介素-1β（IL-1β）、白细胞介素-4（IL-4）、干扰素-γ（IFN-γ）和肿瘤坏死因子α（TNF-α）在二硝基氯苯（DNCB）诱导的小鼠耳部肿胀试验中不同组织中的表达情况，并进一步评估不同组织中细胞因子表达与耳部肿胀程度的相关性。
将小鼠背部用 0.50% DNCB 溶液致敏 3 天。7 天后，测量其耳部厚度并分组。在每组小鼠的左耳中给予不同浓度的 DNCB 溶液刺激，右耳作为对照。每 24 小时测量双耳厚度，刺激后 72 小时处死小鼠。采用酶联免疫吸附试验分别检测小鼠血清、淋巴结和耳部组织中 IL-1β、IL-4、IFN-γ和 TNF-α的表达。
小鼠侧耳肿胀指数与 DNCB 刺激浓度呈线性正相关（r=0.96，p<0.01）。小鼠耳部组织中 IL-1β和 IL-4 的高表达与刺激后 48 小时的耳部肿胀指数呈正相关，相关系数为 0.78（p<0.01）。此外，IFN-γ和 TNF-α与刺激后 48 小时的耳部肿胀指数无显著相关性。
小鼠耳部肿胀程度与 DNCB 浓度及耳部组织中 IL-1β和 IL-4 的表达呈正相关，接触过敏存在亚临床皮肤敏感状态。
