Department of Biochemistry and Molecular Biology, Nihon University School of Dentistry at Matsudo, 2-870-1 Sakaecho-nishi, Matsudo, Chiba 271-8587, Japan.
Dent Mater J. 2011;30(1):58-65. doi: 10.4012/dmj.2010-058. Epub 2011 Jan 26.
β-TCP was implanted into bone defects of dog mandibles, and gene expression profiles were examined using DNA microarray. An implant drill was used to make bone defects, and then β-TCP was filled into bone defects. All specimens were taken out, total RNA was isolated, and levels were analyzed using Affymetrix GeneChip. Higher mRNA levels of connexin 43 (Cx43) and Cx45 were observed in β-TCP-implanted samples compared with controls. The enhancement of Cx43 and Cx45 by β-TCP was confirmed by RT-PCR and real-time RT-PCR. Since Cx43 is known to express in bone-forming regions and is involved in osteogenesis through gap junctional intercellular bone-cell communication (GJIB), immunohistochemical staining was also examined and demonstrated Cx43 protein expression was increased in β-TCP-implanted bone. Cx43 plays a role in osteogenesis through GJIB; therefore, the stimulation of Cx43 expression by β-TCP might be a mechanism of accelerating wound healing and bone formation.
β-TCP 被植入犬下颌骨的骨缺损中，并使用 DNA 微阵列检查基因表达谱。使用植入钻头制作骨缺损，然后将 β-TCP 填充到骨缺损中。取出所有标本，分离总 RNA，并使用 Affymetrix GeneChip 进行分析。与对照组相比，β-TCP 植入样本中连接蛋白 43（Cx43）和 Cx45 的 mRNA 水平更高。通过 RT-PCR 和实时 RT-PCR 证实了 β-TCP 对 Cx43 和 Cx45 的增强作用。由于 Cx43 已知在成骨区域表达，并通过缝隙连接细胞间骨细胞通讯（GJIB）参与成骨，因此还进行了免疫组织化学染色，结果表明 β-TCP 植入的骨中 Cx43 蛋白表达增加。Cx43 通过 GJIB 在成骨中发挥作用；因此，β-TCP 对 Cx43 表达的刺激可能是加速伤口愈合和骨形成的机制。
