Connexin 43 expression at an early stage in dog mandibles by β-TCP.

β-TCP was implanted into bone defects of dog mandibles, and gene expression profiles were examined using DNA microarray. An implant drill was used to make bone defects, and then β-TCP was filled into bone defects. All specimens were taken out, total RNA was isolated, and levels were analyzed using Affymetrix GeneChip. Higher mRNA levels of connexin 43 (Cx43) and Cx45 were observed in β-TCP-implanted samples compared with controls. The enhancement of Cx43 and Cx45 by β-TCP was confirmed by RT-PCR and real-time RT-PCR. Since Cx43 is known to express in bone-forming regions and is involved in osteogenesis through gap junctional intercellular bone-cell communication (GJIB), immunohistochemical staining was also examined and demonstrated Cx43 protein expression was increased in β-TCP-implanted bone. Cx43 plays a role in osteogenesis through GJIB; therefore, the stimulation of Cx43 expression by β-TCP might be a mechanism of accelerating wound healing and bone formation.