Kim Su-Hwan, Kim Kyoung-Hwa, Seo Byoung-Moo, Koo Ki-Tae, Kim Tae-Il, Seol Yang-Jo, Ku Young, Rhyu In-Chul, Chung Chong-Pyoung, Lee Yong-Moo
Department of Periodontology and Dental Research Institute, School of Dentistry, Seoul National University, Seoul, Korea.
J Periodontol. 2009 Nov;80(11):1815-23. doi: 10.1902/jop.2009.090249.
The present study was undertaken to evaluate the potential of periodontal ligament stem cells (PDLSCs) and bone marrow SCs (BMSCs) on alveolar bone regeneration in a canine peri-implant defect model.
Four adult, male beagle dogs were used in this study. Autologous BMSCs from the iliac crests and PDLSCs from extracted teeth were cultured. Three months after extraction, BMSC- and PDLSC-loaded hydroxyapatite/beta-tricalcium phosphate (HA/TCP) (test groups) and cell-free HA/TCP (control group) were implanted in three rectangular, saddle-like peri-implant defects, respectively. The left side of the mandible was initially prepared, and after 8 weeks, the right side was also prepared. The animals were sacrificed after an 8-week healing period. Undecalcified ground sections were prepared. New bone formation and bone-to-implant contact (BIC) were measured histomorphometrically. BMSCs and PDLSCs were fluorescently labeled and traced.
Alveolar bone regeneration in surgically created peri-implant saddle-like defects was more effective in test groups than the control group. The BMSC group had the highest new bone formation (34.99% and 40.17% at healing times of 8 and 16 weeks, respectively) followed by the PDLSC group (31.90% and 36.51%) and control group (23.13% and 28.36%), respectively. Test groups exhibited a significantly higher new bone formation than the control group at 8 weeks, but the same was true for only the BMSC group at 16 weeks (P <0.05). Fluorescently labeled cells were identified adjacent to HA/TCP carriers and, partly, near connective tissues and osteoids.
This study demonstrated the feasibility of using stem cell-mediated bone regeneration to treat peri-implant defects.
本研究旨在评估牙周膜干细胞（PDLSCs）和骨髓干细胞（BMSCs）在犬种植体周围缺损模型中促进牙槽骨再生的潜力。
本研究使用了4只成年雄性比格犬。培养来自髂嵴的自体BMSCs和来自拔除牙齿的PDLSCs。拔牙3个月后，将负载BMSC和PDLSC的羟基磷灰石/β-磷酸三钙（HA/TCP）（试验组）和无细胞HA/TCP（对照组）分别植入三个矩形、鞍状种植体周围缺损处。最初对下颌骨左侧进行处理，8周后对右侧也进行处理。在8周的愈合期后处死动物。制备不脱钙磨片。通过组织形态计量学测量新骨形成和骨-种植体接触（BIC）。对BMSCs和PDLSCs进行荧光标记并追踪。
在手术造成的种植体周围鞍状缺损中，试验组的牙槽骨再生比对照组更有效。BMSC组新骨形成率最高（在8周和16周愈合时分别为34.99%和40.17%），其次是PDLSC组（31.90%和36.51%），对照组分别为（23.13%和28.36%）。试验组在8周时新骨形成明显高于对照组，但在16周时仅BMSC组如此（P<0.05）。在HA/TCP载体附近以及部分在结缔组织和类骨质附近发现了荧光标记的细胞。
本研究证明了使用干细胞介导的骨再生治疗种植体周围缺损的可行性。
