Kim Yeon-Ju, Kim Myung Kyum, Weon Hang-Yeon, Kim Ho-Bin, Yang Deok-Chun
Department of Oriental Medicinal Material & Processing, College of Life Science, Kyung Hee University, Yongin-si, Kyunggi-do, South Korea.
J Gen Appl Microbiol. 2010;56(6):419-25. doi: 10.2323/jgam.56.419.
A Gram-negative, rod-shaped, non-spore-forming and motile bacterial strain TR7-01(T) was isolated from a compost soil in South Korea and subjected to a polyphasic taxonomic study. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain TR7-01(T) belonged to the genus Hydrogenophaga within the class Betaproteobacteria. Strain TR7-01(T) exhibited 16S rRNA gene sequence similarity values of 95.0-98.3% to members of the genus Hydrogenophaga: Hydrogenophaga bisanensis DSM12412(T) (98.3%), Hydrogenophaga flava DSM 619(T) (97.1%), Hydrogenophaga pseudoflava ATCC 33668(T) (96.8%), Hydrogenophaga intermedia S1(T) (96.4%), Hydrogenophaga atypica BSB 41.8(T) (95.8%), Hydrogenophaga defluvii BSB 9.5(T) (95.7%), Hydrogenophaga palleronii CCUG 20334(T) (95.6%), Hydrogenophaga caeni EMB71(T) (95.4%) and Hydrogenophaga taeniospiralis ATCC 49743(T) (95.0%). Chemotaxonomic data revealed that strain TR7-01(T) possesses ubiquinone Q-8, the G+C content was 69.9 mol%, and the predominant fatty acids were 16 : 1 ω7c/15 : 0 iso 2OH, 18 : 1 ω7c/ω9t/ω12t and C(16:0), all of which corroborated our assignment of the strain to the genus Hydrogenophaga. The results of DNA-DNA hybridization and physiological and biochemical tests clearly demonstrated that strain TR7-01(T) represents a distinct species. Based on these data, TR7-01(T) (= KCTC 12203(T) = DSM 18117(T)) should be classified as a novel Hydrogenophaga species, for which the name Hydrogenophaga temperata sp. nov. has been proposed.
从韩国堆肥土壤中分离出一株革兰氏阴性、杆状、无芽孢且具运动性的细菌菌株TR7-01(T)，并对其进行了多相分类研究。基于16S rRNA基因序列的系统发育分析表明，菌株TR7-01(T)属于β-变形菌纲中的嗜氢菌属。菌株TR7-01(T)与嗜氢菌属成员的16S rRNA基因序列相似性值为95.0 - 98.3%：双山嗜氢菌DSM12412(T)（98.3%）、黄嗜氢菌DSM 619(T)（97.1%）、假黄嗜氢菌ATCC 33668(T)（96.8%）、中间嗜氢菌S1(T)（96.4%）、非典型嗜氢菌BSB 41.8(T)（95.8%）、污水嗜氢菌BSB 9.5(T)（95.7%）、帕氏嗜氢菌CCUG 20334(T)（95.6%）、嗜氢菌EMB71(T)（95.4%）和旋形嗜氢菌ATCC 49743(T)（95.0%）。化学分类数据显示，菌株TR7-01(T)含有泛醌Q-8，G+C含量为69.9 mol%，主要脂肪酸为16 : 1 ω7c/15 : 0 iso 2OH、18 : 1 ω7c/ω9t/ω12t和C(16:0)，所有这些都证实了我们将该菌株归为嗜氢菌属的分类。DNA-DNA杂交以及生理生化测试结果清楚地表明，菌株TR7-01(T)代表一个独特的物种。基于这些数据，TR7-01(T)（= KCTC 12203(T) = DSM 18117(T)）应被分类为嗜氢菌属的一个新物种，为此已提议将其命名为嗜温嗜氢菌（Hydrogenophaga temperata sp. nov.）。
