AceEMzyme Co., Ltd., Academic Industry Cooperation, 327 Chungang-no Anseong-si, Kyonggi-do 17579, Republic of Korea.
Department of Biotechnology, Hankyong National University, 327 Chungang-no Anseong-si, Kyonggi-do 17579, Republic of Korea.
Int J Syst Evol Microbiol. 2020 Jan;70(1):555-561. doi: 10.1099/ijsem.0.003787.
A novel Gram-stain-negative, yellowish-pigmented bacterial strain, designated LA-38, was isolated from activated sludge of wastewater treatment plants in Hanam city, South Korea. Cell of LA-38 were rod-shaped, aerobic, motile and non-spore-forming. In phylogenetic analyses based on 16S rRNA genes, LA-38 clustered with species of the genus and appeared closely related to DSM 5680 (99.2 % similarity), DSM 63 (98.2 %), KCTC 42478 (98.1 %), DSM 15342 (98.1 %), DSM 15341 (98.0 %) and DSM 2082 (97.2 %). The average nucleotide identities between LA-38 and the closely related strains were 79.3-88.5 %, indicating that LA-38 represents a novel species of the genus . The DNA G+C content of the genomic DNA was 69.9 mol% and ubiquinone Q-8 was the predominant respiratory quinone. The major cellular fatty acids (>5 %) were C, cyclo-C, Cω7 and/or Cω6 (summed feature 3), and Cω7 and/or Cω6 (summed feature 8). The major polar lipids consisted of phosphatidylglycerol, diphosphatidylglycerol and phosphatidylethanolamine, the major polyamines were 2-hydroxyputrescine and putrescine. ANI calculation, physiological and biochemical characteristics indicated that LA-38 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is LA-38 (=KACC 19730=LMG 30805).
一株新型革兰氏阴性、浅黄色着色的细菌菌株，被命名为 LA-38，从韩国华城市污水处理厂的活性污泥中分离得到。LA-38 的细胞呈杆状，需氧，能动，不产芽孢。在基于 16S rRNA 基因的系统发育分析中，LA-38 与属内的种聚在一起，与 DSM 5680（99.2%相似性）、DSM 63（98.2%）、KCTC 42478（98.1%）、DSM 15342（98.1%）、DSM 15341（98.0%）和 DSM 2082（97.2%）最为接近。LA-38 与密切相关的菌株之间的平均核苷酸同一性为 79.3-88.5%，表明 LA-38 代表了属内的一个新种。基因组 DNA 的 G+C 含量为 69.9mol%，主要呼吸醌为醌-8。主要的细胞脂肪酸（>5%）为 C、环-C、Cω7 和/或 Cω6（特征 3 总和）和 Cω7 和/或 Cω6（特征 8 总和）。主要的极性脂由磷脂酰甘油、双磷脂酰甘油和磷脂酰乙醇胺组成，主要的多胺为 2-羟腐胺和腐胺。ANI 计算、生理生化特征表明，LA-38 代表了属内的一个新种，建议将其命名为 sp. nov.。其模式菌株为 LA-38（=KACC 19730=LMG 30805）。
