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sp. nov.,从活性污泥中分离得到。

sp. nov., isolated from activated sludge.

机构信息

AceEMzyme Co., Ltd., Academic Industry Cooperation, 327 Chungang-no Anseong-si, Kyonggi-do 17579, Republic of Korea.

Department of Biotechnology, Hankyong National University, 327 Chungang-no Anseong-si, Kyonggi-do 17579, Republic of Korea.

出版信息

Int J Syst Evol Microbiol. 2020 Jan;70(1):555-561. doi: 10.1099/ijsem.0.003787.

Abstract

A novel Gram-stain-negative, yellowish-pigmented bacterial strain, designated LA-38, was isolated from activated sludge of wastewater treatment plants in Hanam city, South Korea. Cell of LA-38 were rod-shaped, aerobic, motile and non-spore-forming. In phylogenetic analyses based on 16S rRNA genes, LA-38 clustered with species of the genus and appeared closely related to DSM 5680 (99.2 % similarity), DSM 63 (98.2 %), KCTC 42478 (98.1 %), DSM 15342 (98.1 %), DSM 15341 (98.0 %) and DSM 2082 (97.2 %). The average nucleotide identities between LA-38 and the closely related strains were 79.3-88.5 %, indicating that LA-38 represents a novel species of the genus . The DNA G+C content of the genomic DNA was 69.9 mol% and ubiquinone Q-8 was the predominant respiratory quinone. The major cellular fatty acids (>5 %) were C, cyclo-C, Cω7 and/or Cω6 (summed feature 3), and Cω7 and/or Cω6 (summed feature 8). The major polar lipids consisted of phosphatidylglycerol, diphosphatidylglycerol and phosphatidylethanolamine, the major polyamines were 2-hydroxyputrescine and putrescine. ANI calculation, physiological and biochemical characteristics indicated that LA-38 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is LA-38 (=KACC 19730=LMG 30805).

摘要

一株新型革兰氏阴性、浅黄色着色的细菌菌株,被命名为 LA-38,从韩国华城市污水处理厂的活性污泥中分离得到。LA-38 的细胞呈杆状,需氧,能动,不产芽孢。在基于 16S rRNA 基因的系统发育分析中,LA-38 与属内的种聚在一起,与 DSM 5680(99.2%相似性)、DSM 63(98.2%)、KCTC 42478(98.1%)、DSM 15342(98.1%)、DSM 15341(98.0%)和 DSM 2082(97.2%)最为接近。LA-38 与密切相关的菌株之间的平均核苷酸同一性为 79.3-88.5%,表明 LA-38 代表了属内的一个新种。基因组 DNA 的 G+C 含量为 69.9mol%,主要呼吸醌为醌-8。主要的细胞脂肪酸(>5%)为 C、环-C、Cω7 和/或 Cω6(特征 3 总和)和 Cω7 和/或 Cω6(特征 8 总和)。主要的极性脂由磷脂酰甘油、双磷脂酰甘油和磷脂酰乙醇胺组成,主要的多胺为 2-羟腐胺和腐胺。ANI 计算、生理生化特征表明,LA-38 代表了属内的一个新种,建议将其命名为 sp. nov.。其模式菌株为 LA-38(=KACC 19730=LMG 30805)。

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